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Recently, we abrogated the F2 adult phenotype with essential nutrient supplementation (ENS) of intermediates along the 1\u2010carbon pathway. With the use of the same grandparental uterine artery ligation model, we profiled the F2 serum metabolome at weaning [postnatal day (d)21;\n                    <jats:italic>n<\/jats:italic>\n                    = 76] and adulthood (d160;\n                    <jats:italic>n<\/jats:italic>\n                    = 12) to test if MetS is preceded by alterations in the metabolome. Indicative of developmentally programmed MetS, adult F2, formerly IUGR rats, were obese (621\n                    <jats:italic>vs.<\/jats:italic>\n                    461 g;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.0001), dyslipidemic (133\n                    <jats:italic>vs.<\/jats:italic>\n                    67 mg\/dl;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001), and glucose intolerant (26\n                    <jats:italic>vs.<\/jats:italic>\n                    15 mg\/kg\/min;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01). Unbiased gas chromatography\u2010mass spectrometry (GC\u2010MS) profiling revealed 34 peaks corresponding to 12 nonredundant metabolites and 9 unknowns to be changing at weaning [false discovery rate (FDR) &lt; 0.05]. Markers of later\u2010in\u2010life MetS included citric acid, glucosamine, myoinositol, and proline (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.03). Hierarchical clustering revealed grouping by IUGR lineage and supplementation at d21 and d160. Weanlings grouped distinctly for ENS and IUGR by partial least\u2010squares discriminate analysis (PLS\u2010DA;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01), whereas paternal andmaternal IUGR (IUGR\n                    <jats:sup>pat<\/jats:sup>\n                    \/IUGR\n                    <jats:sup>mat<\/jats:sup>\n                    , respectively) control\u2010fed rats, destined for MetS, had a distinct metabolome at weaning (randomForest analysis; class error &lt; 0.1) and adulthood (PLS\u2010DA;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). In sum, we have found that alterations in the metabolome accompany heritable IUGR, precede adult\u2010onset MetS, and are partially amenable to dietary intervention.\u2014 Seferovic, M. D., Goodspeed, D. M., Chu, D. M., Krannich, L. A., Gonzalez\u2010Rodriguez, P. J., Cox, J. E., Aagaard, K. M. Heritable IUGR and adult metabolic syndrome are reversible and associated with alterations in the metabolome following dietary supplementation of one\u2010carbon intermediates.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    29, 2640\u20102652 (2015).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.14-266387","type":"journal-article","created":{"date-parts":[[2015,3,10]],"date-time":"2015-03-10T22:52:23Z","timestamp":1426027943000},"page":"2640-2652","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Heritable IUGR and adult metabolic syndrome are reversible and associated with alterations in the metabolome following dietary supplementation of 1\u2010carbon intermediates"],"prefix":"10.1096","volume":"29","author":[{"given":"Maxim D.","family":"Seferovic","sequence":"first","affiliation":[{"name":"Departments of Obstetrics and Gynecology Division of Maternal\u2010Fetal Medicine Baylor College of Medicine Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle M.","family":"Goodspeed","sequence":"additional","affiliation":[{"name":"Departments of Obstetrics and Gynecology Division of Maternal\u2010Fetal Medicine Baylor College of Medicine Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derrick M.","family":"Chu","sequence":"additional","affiliation":[{"name":"Interdepartmental Program in Translational Biology and Molecular Medicine Baylor College of Medicine Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura A.","family":"Krannich","sequence":"additional","affiliation":[{"name":"Departments of Obstetrics and Gynecology Division of Maternal\u2010Fetal Medicine Baylor College of Medicine Houston Texas 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