{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T19:17:00Z","timestamp":1792264620054,"version":"4.4.0"},"reference-count":58,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 2009\u2010290"],"award-info":[{"award-number":["PIP 2009\u2010290"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Lectin\u2010glycan recognition systems play central roles in many physiologic and pathologic processes. We identified a role for galectin\u20101 (Gal\u20101), a highly conserved glycan\u2010binding protein, in the control of sperm function. We found that Gal\u20101 is expressed in the epididymis and associates with sperm during epididymal maturation. Exposure of sperm to Gal\u20101 resulted in glycan\u2010dependent modulation of the acrosome reaction (AR), a key event in the fertilization process. Gal\u20101\u2010deficient (\n                    <jats:italic>Lgals1<\/jats:italic>\n                    <jats:sup>\u2010\/\u2010<\/jats:sup>\n                    )mice revealed the essential contribution of this lectin for full sperm fertilizing ability both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo.<\/jats:italic>\n                    Mechanistically,\n                    <jats:italic>Lgals1<\/jats:italic>\n                    <jats:sup>\u2010\/\u2010<\/jats:sup>\n                    sperm exhibited defects in their ability to develop hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover,\n                    <jats:italic>Lgals1<\/jats:italic>\n                    <jats:sup>\u2010\/\u2010<\/jats:sup>\n                    sperm showed a decreased ability to control cell volume and to undergo progesterone\u2010induced AR, phenotypes that were rescued by exposure of the cells to recombinant Gal\u20101. Interestingly, the AR defect was associated with a deficiency in sperm membrane potential hyperpolarization. Our study highlights the relevance of the Gal\u20101\u2010glycan axis in sperm function with critical implications in mammalian reproductive biology.\u2014Vasen, G., Battistone, M. A., Croci, D. O., Brukman, N. G., Weigel Mu\u00f1oz, M., Stupirski, J. C., Rabinovich, G. A., Cuasnic\u00fa, P. S. The galectin\u20101\u2010glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    29, 4189\u20104200 (2015).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.15-270975","type":"journal-article","created":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T20:32:55Z","timestamp":1435782775000},"page":"4189-4200","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The galectin\u20101\u2010glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization"],"prefix":"10.1096","volume":"29","author":[{"given":"Gustavo","family":"Vasen","sequence":"first","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME\u2010CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Agustina","family":"Battistone","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME\u2010CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego O.","family":"Croci","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME\u2010CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00e1s G.","family":"Brakman","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME\u2010CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Weigel","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME\u2010CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"Stupirski","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME\u2010CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel A.","family":"Rabinovich","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME\u2010CONICET) Buenos Aires Argentina"},{"name":"Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia S.","family":"Cuasnic\u00fa","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME\u2010CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio785"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705134200"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1559-0275-11-10"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.11.7731"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.22.9920-9929.2004"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0917"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R600001200"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413569200"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.004291"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201404025"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2013.01.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2007.09.002"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2011.05.001"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod55.3.548"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.006361"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gah144"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2011.09.003"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028514"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2012.214"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1303707110"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1680"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gas043"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cws104"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.06.017"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod13.5.513"},{"key":"e_1_2_7_27_1","first-page":"145","article-title":"Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. 1979","volume":"24","author":"Towbin H.","year":"1992","journal-title":"Biotechnology"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.01.043"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.1.436"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1772"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.056770"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100240108"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a019295"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028359"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dev002"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb02101.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.114.127266"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2008.00611.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.4.1062"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(92)90137-6"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.393488"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809233105"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-8691-8"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414140200"},{"key":"e_1_2_7_45_1","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1093\/glycob\/cwh101","article-title":"Effects of galectin\u20101 on regulation of progesterone production in granulosa cells from pig ovaries in vitro","volume":"14","author":"Walzel H.","year":"2004","journal-title":"Glycobiology"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.01.005"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802981"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119.4.1229"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-007-7037-7"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.071928"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.169"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24166"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod67.1.269"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1018202108"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803223105"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2013.269597"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.02.005"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1111104108"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1423869112"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.15-270975","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.15-270975","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.15-270975","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T01:31:56Z","timestamp":1761701516000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.15-270975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7]]},"references-count":58,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1096\/fj.15-270975"],"URL":"https:\/\/doi.org\/10.1096\/fj.15-270975","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7]]},"assertion":[{"value":"2015-07-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}