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Recent studies indicate that tankyrases (TNKSs), which poly(ADP\u2010ribosyl)ate target proteins and direct them toward proteasomal degradation, affect overall metabolism, but the exact molecular mechanisms remain unclear. We used zebrafish larvae as a model to study the mechanisms by which\n                    <jats:italic>TNKS1b<\/jats:italic>\n                    , the zebrafish ortholog of mammalian\n                    <jats:italic>TNKS1<\/jats:italic>\n                    , regulates glucose homeostasis and somatic growth.\n                    <jats:italic>In situ<\/jats:italic>\n                    hybridization revealed that\n                    <jats:italic>TNKS1b<\/jats:italic>\n                    mRNA is prominently expressed in the hypothalamus and pituitary of the embryonic and larval brain. In the pituitary,\n                    <jats:italic>TNKS1b<\/jats:italic>\n                    is coexpressed with pro\u2010opiomelanocortin a (\n                    <jats:italic>pomca<\/jats:italic>\n                    ) gene in corticotropes and melanotropes. Knockdown of\n                    <jats:italic>TNKS1b<\/jats:italic>\n                    reduced the linear growth of the larvae, stimulated insulin gene and glucose transporter 4 protein, and suppressed gluconeogenic phosphoenolpyruvate carboxykinase 1 gene. This result indicates rapid glucose utilization and reduction of gluconeogenesis in\n                    <jats:italic>TNKS1b<\/jats:italic>\n                    \u2010deficient larvae. Knockdown of\n                    <jats:italic>TNKS1b<\/jats:italic>\n                    down\u2010regulated\n                    <jats:italic>pomca<\/jats:italic>\n                    expression and diminished \u03b1\u2010melanocyte\u2010stimulating hormone in the pars intermedia. Furthermore, down\u2010regulation of\n                    <jats:italic>TNKS1b<\/jats:italic>\n                    suppressed the expression of melanocortin receptor 3 and increased the expression of melanocortin receptor 4. The collective data suggest that\n                    <jats:italic>TNKS1b<\/jats:italic>\n                    modulates glucoregulatory mechanisms and the somatic growth of zebrafish larvae\n                    <jats:italic>via<\/jats:italic>\n                    the central melanocortin system.\u2014Wang, H., Semenova, S., Kuusela, S., Panula, P., Lehtonen, S. Tankyrases regulate glucoregulatory mechanisms and somatic growth\n                    <jats:italic>via<\/jats:italic>\n                    the central melanocortin system in zebrafish larvae.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    29, 4435\u20104448 (2015).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.15-271817","type":"journal-article","created":{"date-parts":[[2015,7,13]],"date-time":"2015-07-13T22:50:52Z","timestamp":1436827852000},"page":"4435-4448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tankyrases regulate glucoregulatory mechanisms and somatic growth\n                    <i>via<\/i>\n                    the central melanocortin system in zebrafish larvae"],"prefix":"10.1096","volume":"29","author":[{"given":"Hong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Semenova","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Anatomy and Neuroscience Centre University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Kuusela","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pertti","family":"Panula","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Anatomy and Neuroscience Centre University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanna","family":"Lehtonen","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Helsinki Helsinki Finland"},{"name":"Institute of Biomedicine, Anatomy and Neuroscience Centre University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2011.06248.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-012-8240-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1455"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevNeurobiol.v11.i1.30"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.11.042"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2006-0034"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81865-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.9.7791"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/509"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/2407"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.011346"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.03.039"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21447"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-7503com"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.12.014"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1232995"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1677\/JME-06-0037"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2010.10.010"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.03.040"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.53.2.486"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000916"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20060793"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2012.52"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.2337\/db08-1781"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2011.08.007"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2011.02.026"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2012.0841"},{"key":"e_1_2_7_29_1","volume-title":"The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Danio rerio)","author":"Westerfield M.","year":"2000"},{"key":"e_1_2_7_30_1","first-page":"1229","article-title":"Zebrafish whole mount high\u2010resolution double fluorescent in situ hybridization","author":"Brend T.","year":"2009","journal-title":"J. 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