{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:54:12Z","timestamp":1791208452374,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T00:00:00Z","timestamp":1440374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Neisseria<\/jats:italic>\n                    adhesin A (NadA) is one of the antigens of Bexsero, the recently licensed multicomponent vaccine against serogroup B\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    (MenB). NadA belongs to the class of oligomeric coiled\u2010coil adhesins and is able to mediate adhesion and invasion of human epithelial cells. As a vaccine antigen, NadA has been shown to induce high levels of bactericidal antibodies; however, the domains important for protective response are still unknown. In order to further investigate its immunogenic properties, we have characterized the murine IgG1 mAb (6E3) that was able to recognize the 2 main antigenic variants of NadA on the surface of MenB strains. The epitope targeted by mAb 6E3 was mapped by hydrogen\u2010deuterium exchange mass spectrometry and shown to be located on the coiled\u2010coil stalk region of NadA (aa 206\u2010249). Although no serum bactericidal activity was observed for murine IgG1 mAb 6E3, functional activity was restored when using chimeric antibodies in which the variable regions of the murine mAb 6E3 were fused to human IgG3 constant regions, thus confirming the protective nature of the mAb 6E3 epitope. The use of chimeric antibody molecules will enable future investigations of complement\u2010mediated antibody functionality independently of the Fc\u2010mediated differences in complement activation.\u2014Bertoldi, I., Faleri, A., Galli, B., Lo Surdo, P., Liguori, A., Norais, N., Santini, L., Masignani, V., Pizza, M., Giuliani, M. M. Exploiting chimeric human antibodies to characterize a protective epitope of\n                    <jats:italic>Neisseria<\/jats:italic>\n                    adhesin A, one of the Bexsero vaccine components.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    30, 93\u2010101 (2016).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.15-273813","type":"journal-article","created":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T21:59:52Z","timestamp":1440453592000},"page":"93-101","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Exploiting chimeric human antibodies to characterize a protective epitope of\n                    <i>Neisseria<\/i>\n                    adhesin A, one of the Bexsero vaccine components"],"prefix":"10.1096","volume":"30","author":[{"given":"Isabella","family":"Bertoldi","sequence":"first","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnese","family":"Faleri","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Galli","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola Lo","family":"Surdo","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Liguori","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Norais","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Santini","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vega","family":"Masignani","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariagrazia","family":"Pizza","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzia Monica","family":"Giuliani","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GlaxoSmithKline Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603940103"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020407"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04423.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000710"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01085-12"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/424464"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.7.4052-4060.2004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.05.049"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.7.4217-4223.2004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00825-13"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.02.098"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00936-09"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00027-10"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1419686111"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.21.6851"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(06)90003-0"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/PPO.0b013e318173a5d5"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbt.2011.03.021"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2006.07.025"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.01.099"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2005.09.034"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(99)00184-2"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.2.1151-1160.2005"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.12.8.970-976.2005"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.2.5.745"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1222845110"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300656w"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3002234"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-012-9782-0"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.09.046"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05956-11"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb08712.x"},{"key":"e_1_2_8_34_1","first-page":"115","article-title":"Human immunoglobulin subclasses","volume":"5","author":"Normansell D. E.","year":"1987","journal-title":"Diagn. Clin. Immunol."},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80094-8"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06161-11"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704270200"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.15-273813","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.15-273813","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.15-273813","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T19:58:13Z","timestamp":1761681493000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.15-273813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,24]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1096\/fj.15-273813"],"URL":"https:\/\/doi.org\/10.1096\/fj.15-273813","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,24]]},"assertion":[{"value":"2015-04-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}