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The angiotensin converting enzyme inhibitor lisinopril and mineralocorticoid receptor (MR) antagonist spironolactone indirectly and directly target MR. The presence and function of MR in skeletal muscle have not been explored. MR mRNA and protein are present in all tested skeletal muscles from both wild\u2010type mice and DMD mouse models. MR expression is cell autonomous in both undifferentiated myoblasts and differentiated myotubes from mouse and human skeletal muscle cultures. To test for MR function in skeletal muscle, global gene expression analysis was conducted on human myotubes treated with MR agonist (aldosterone; EC50 1.3 nM) or antagonist (spironolactone; IC50 1.6 nM), and 53 gene expression differences were identified. Five differences were conserved in quadriceps muscles from dystrophic mice treated with spironolactone plus lisinopril (IC\n                    <jats:sub>50<\/jats:sub>\n                    0.1 nM) compared with untreated controls. Genes down\u2010regulated more than 2\u2010fold by MR antagonism included\n                    <jats:italic>FOS, ANKRD1<\/jats:italic>\n                    , and\n                    <jats:italic>GADD45B<\/jats:italic>\n                    , with known roles in skeletal muscle, in addition to\n                    <jats:italic>NPR3<\/jats:italic>\n                    and\n                    <jats:italic>SERPINA3, bona fide<\/jats:italic>\n                    targets of MR in other tissues. MR is a novel drug target in skeletal muscle and use of clinically safe antagonists may be beneficial for muscle diseases.\u2014 Chadwick, J. A., Hauck, J. S., Lowe, J., Shaw, J. J., Guttridge, D. C., Gomez\u2010Sanchez, C. E., Gomez\u2010Sanchez, E. P., Rafael\u2010Fortney, J. A. Mineralocorticoid receptors are present in skeletal muscle and represent a potential therapeutic target.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    29, 4544\u20104554 (2015).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.15-276782","type":"journal-article","created":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T22:40:16Z","timestamp":1437000016000},"page":"4544-4554","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Mineralocorticoid receptors are present in skeletal muscle and represent a potential therapeutic target"],"prefix":"10.1096","volume":"29","author":[{"given":"Jessica A.","family":"Chadwick","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biochemistry The Ohio State University Columbus Ohio USA"},{"name":"Department of Physiology and Cell Biology The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Spencer","family":"Hauck","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biochemistry The Ohio State University Columbus Ohio USA"},{"name":"Department of Physiology and Cell Biology The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeovanna","family":"Lowe","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biochemistry The Ohio State University Columbus Ohio USA"},{"name":"Department of Physiology and Cell Biology The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremiah J.","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biochemistry The Ohio State University Columbus Ohio USA"},{"name":"Department of Physiology and Cell Biology The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis C.","family":"Guttridge","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Immunology Medical Genetics College of Medicine The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso E.","family":"Gomez\u2010Sanchez","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of Mississippi Medical Center Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise P.","family":"Gomez\u2010Sanchez","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Mississippi Medical Center Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill A.","family":"Rafael\u2010Fortney","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biochemistry The Ohio State University Columbus Ohio USA"},{"name":"Department of Physiology and Cell Biology The Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23528"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07815-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1987.00520200022012"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurjhf\/hfp028"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00296340"},{"key":"e_1_2_7_7_1","first-page":"9","article-title":"Old and new therapeutic developments in steroid treatment in Duchenne muscular dystrophy","volume":"31","author":"Angelini C.","year":"2012","journal-title":"Acta Myol."},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.031716"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2007.08.029"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.06.038"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2217\/cer.12.5"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2014.04.005"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1527-3466.2001.tb00064.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3233\/JND-150099"},{"key":"e_1_2_7_15_1","first-page":"179","article-title":"Human steroid receptor sanderb\u2010A gene products form a superfamily of enhancer\u2010binding proteins","volume":"5","author":"Weinberger C.","year":"1987","journal-title":"Clin. 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