{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T07:54:00Z","timestamp":1793606040427,"version":"4.5.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,2,12]],"date-time":"2016-02-12T00:00:00Z","timestamp":1455235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL129853"],"award-info":[{"award-number":["HL129853"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>\n                    Platelet\u2010derived exosomes mediate platelet atherogenic interactions with endothelial cells and monocytes. A new method for isolation of plasma platelet\u2010derived exosomes is described and used to examine effects of aging and aspirin on exosome cargo proteins. Exosome secretion by purified platelets\n                    <jats:italic>in vitro<\/jats:italic>\n                    did not increase after exposure to thrombin or collagen, as assessed by exosome counts and quantification of the CD81 exosome marker. Thrombin and collagen increased exosome content of \u03b1\u2010granule chemokines CXCL4 and CXCL7 and cytoplasmic high\u2010mobility group box 1 (HMGB1) protein, but not membrane platelet glycoprotein VI (GPVI), with dependence on extracellular calcium. Aspirin consumption significantly blocked thrombin\u2010 and collagen\u2010induced increases in exosome cargo levels of chemokines and HMGB1, without altering total exosome secretion or GPVI cargo. Plasma platelet\u2010derived exosomes, enriched by absorption with mouse antihuman CD42b [platelet glycoprotein Ib (GPIb)] mAb, had sizes and cargo protein contents similar to those of exosomes from purified platelets. The plasma platelet\u2010derived exosome number is lower and its chemokine and HMGB1 levels higher after age 65 yr. Aspirin consumption significantly suppressed cargo protein levels of plasma platelet\u2010derived exosomes without altering total levels of exosomes. Cargo proteins of human plasma platelet\u2010derived exosomes may biomark platelet abnormalities and\n                    <jats:italic>in vivo<\/jats:italic>\n                    effects of drugs.\u2014Goetzl, E. J., Goetzl, L., Karliner, J. S., Tang, N., Pulliam, L. Human plasma platelet\u2010derived exosomes: effects of aspirin.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    30, 2058\u20132063 (2016).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201500150r","type":"journal-article","created":{"date-parts":[[2016,2,13]],"date-time":"2016-02-13T07:34:13Z","timestamp":1455348853000},"page":"2058-2063","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Human plasma platelet\u2010derived exosomes: effects of aspirin"],"prefix":"10.1096","volume":"30","author":[{"given":"Edward J.","family":"Goetzl","sequence":"first","affiliation":[{"name":"Department of Medicine University of California, San Francisco San Francisco California USA"},{"name":"Jewish Home of San Francisco San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Goetzl","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences Temple University Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel S.","family":"Karliner","sequence":"additional","affiliation":[{"name":"Department of Medicine University of California, San Francisco San Francisco California USA"},{"name":"Veterans Affairs Medical Center San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norina","family":"Tang","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn","family":"Pulliam","sequence":"additional","affiliation":[{"name":"Department of Medicine University of California, San Francisco San Francisco California USA"},{"name":"Department of Laboratory Medicine University of California, San Francisco San Francisco California USA"},{"name":"Veterans Affairs Medical Center San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.11.3791"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v3.24692"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2014.04.002"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"294","DOI":"10.3389\/fphys.2014.00294","article-title":"Platelets and their chemokines in atherosclerosis\u2010clinical applications","volume":"5","author":"Von Hundelshausen P.","year":"2014","journal-title":"Front. 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