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In addition to its role in tissue injury, the persistent presence of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    \u2013secreted products may also affect epithelial repair ability, raising the need for new antivirulence therapies. The purpose of our study was to better understand the outcomes of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    exoproducts exposure on airway epithelial repair processes to identify a strategy to counteract their deleterious effect. We found that\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    exoproducts significantly decreased wound healing, migration, and proliferation rates, and impaired the ability of directional migration of primary non\u2013cystic fibrosis (CF) human airway epithelial cells. Impact of exoproducts was inhibited after mutations in\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    genes that encoded for the quorum\u2010sensing (QS) transcriptional regulator, LasR, and the elastase, LasB, whereas impact was restored by LasB induction in \u0394\n                    <jats:italic>lasR<\/jats:italic>\n                    mutants.\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    purified elastase also induced a significant decrease in non\u2010CF epithelial repair, whereas protease inhibition with phosphoramidon prevented the effect of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    exoproducts. Furthermore, treatment of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    cultures with 4\u2010hydroxy\u20102,5\u2010dimethyl\u20103(2H)furanone, a QS inhibitor, abrogated the negative impact of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    exoproducts on airway epithelial repair. Finally, we confirmed our findings in human airway epithelial cells from patients with CF, a disease featuring\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    chronic respiratory infection. These data demonstrate that secreted proteases under the control of the\n                    <jats:italic>lasR<\/jats:italic>\n                    QS system impair airway epithelial repair and that QS inhibitors could be of benefit to counteract the deleterious effect of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    in infected patients.\u2014Ruffin, M., Bilodeau, C., Maill\u00e9, \u00c9., LaFayette, S.L., McKay, G.A., Trinh, N.T.N., Beaudoin, T., Desrosiers, M.\u2010Y., Rousseau, S., Nguyen, D., Brochiero, E. Quorum\u2010sensing inhibition abrogates the deleterious impact of\n                    <jats:italic>Pseudomon asaeruginosa<\/jats:italic>\n                    on airway epithelial repair. FASEBJ. 30, 3011\u20133025 (2016).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201500166r","type":"journal-article","created":{"date-parts":[[2016,5,14]],"date-time":"2016-05-14T01:26:06Z","timestamp":1463189166000},"page":"3011-3025","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Quorum\u2010sensing inhibition abrogates the deleterious impact of\n                    <i>Pseudomonas aeruginosa<\/i>\n                    on airway epithelial repair"],"prefix":"10.1096","volume":"30","author":[{"given":"Manon","family":"Ruffin","sequence":"first","affiliation":[{"name":"Centre de Recherche du Centre Hospitalier de l'Universit\u00e9al (CRCHUM)  Montr\u00e9al Qu\u00e9bec Canada"},{"name":"D\u00e9partement de M\u00e9decine Universit\u00e9 de Montr\u00e9al  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