{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T07:36:56Z","timestamp":1773819416272,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,4,8]],"date-time":"2016-04-08T00:00:00Z","timestamp":1460073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI092117"],"award-info":[{"award-number":["R01AI092117"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Stenosis is a critical problem in the long\u2010term efficacy of tissue\u2010engineered vascular grafts (TEVGs). We previously showed that host monocyte infiltration and activation within the graft drives stenosis and that TGF\u2010\u03b2 receptor 1 (TGF\u2010\u03b2R1) inhibition can prevent it, but the latter effect was attributed primarily to inhibition of mesenchymal cell expansion. In this study, we assessed the effects of TGF\u2010\u03b2R1 inhibition on the host monocytes. Biodegradable TEVGs were implanted as inferior vena cava interposition conduits in 2 groups of C57BL\/6 mice (\n                    <jats:italic>n<\/jats:italic>\n                    = 25\/group): unseeded grafts and unseeded grafts with TGF\u2010\u03b2R1 inhibitor systemic treatment for the first 2 wk. The TGF\u2010\u03b2R1 inhibitor treatment effectively improved TEVG patency at 6 mo compared to the untreated control group (91.7\n                    <jats:italic>vs.<\/jats:italic>\n                    48%,\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001), which is associated with a reduction in classic activation of mononuclear phagocytes. Consistent with these findings, the addition of rTGF\u2010\u03b2 to LPS\/IFN\u2010\u03b3\u2010stimulated monocytes enhanced secretion of inflammatory cytokines TNF\u2010\u03b1, IL\u201012, and IL\u20106; this effect was blocked by TGF\u2010\u03b2R1 inhibition (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.0001). These findings suggest that the TGF\u2010\u03b2 signaling pathway contributes to TEVG stenosis by inducing classic activation of host monocytes. Furthermore, blocking monocyte activation by TGF\u2010\u03b2R1 inhibition provides a viable strategy for preventing TEVG stenosis while maintaining neotissue formation.\u2014Lee, Y.\u2010U., de Dios Ruiz\u2010Rosado, J., Mahler, N., Best, C. A., Tara, S., Yi, T., Shoji, T., Sugiura, T., Lee, A. Y., Robledo\u2010Avila, F., Hibino, N., Pober, J. S., Shinoka, T., Partida\u2010Sanchez, S., Breuer, C. K. TGF\u2010\u03b2 receptor 1 inhibition prevents stenosis of tissue\u2010engineered vascular grafts by reducing host mononuclear phagocyte activation. FASEB J. 30, 2627\u20102636 (2016).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201500179r","type":"journal-article","created":{"date-parts":[[2016,4,9]],"date-time":"2016-04-09T00:07:07Z","timestamp":1460160427000},"page":"2627-2636","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["TGF\u2010\u03b2 receptor 1 inhibition prevents stenosis of tissue\u2010engineered vascular grafts by reducing host mononuclear phagocyte activation"],"prefix":"10.1096","volume":"30","author":[{"given":"Yong\u2010Ung","family":"Lee","sequence":"first","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan de Dios","family":"Ruiz\u2010Rosado","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan","family":"Mahler","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cameron A.","family":"Best","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhei","family":"Tara","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai","family":"Yi","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiro","family":"Shoji","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadahisa","family":"Sugiura","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Avione Y.","family":"Lee","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Robledo\u2010Avila","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narutoshi","family":"Hibino","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"},{"name":"Department of Cardiothoracic Surgery\u2010The Heart Center Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordan S.","family":"Pober","sequence":"additional","affiliation":[{"name":"Department of Immunobiology Yale University New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiharu","family":"Shinoka","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"},{"name":"Department of Cardiothoracic Surgery\u2010The Heart Center Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santiago","family":"Partida\u2010Sanchez","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher K.","family":"Breuer","sequence":"additional","affiliation":[{"name":"Tissue Engineering Program Nationwide Children's Hospital Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911465107"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2009.09.057"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-186585"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.jacc.2014.08.057","article-title":"TGF\u03b2R1 inhibition blocks the formation of stenosis in tissue\u2010engineered vascular grafts","volume":"65","author":"Duncan D. 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