{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:39:09Z","timestamp":1790516349663,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,12,14]],"date-time":"2016-12-14T00:00:00Z","timestamp":1481673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006367","name":"Association Instituts Carnot","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006367","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013340","name":"Institute Pasteur De Lille","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013340","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Angiogenesis is a cause of visual impairment and blindness in the wet form of age\u2010related macular degeneration and in ischemic retinopathies. Current therapies include use of anti\u2010VEGF agents to reduce choroidal neovascularization (CNV) and edema. These treatments are effective in most cases, but spontaneous or acquired resistance to anti\u2010VEGF and possible adverse effects of long\u2010term VEGF inhibition in the retina and choroid highlight a need for additional alternative therapies. Integrins \u03b1v\u03b23 and \u03b1v\u03b25, which regulate endothelial cell proliferation and stabilization, have been implicated in ocular angiogenesis. Lebecetin (LCT) is a 30\u2010kDa heterodimeric C\u2010type lectin that is isolated from\n                    <jats:italic>Macrovipera lebetina<\/jats:italic>\n                    venom and interacts with \u03b15\u03b21\u2010 and \u03b1v\u2010containing integrins. We previously showed that LCT inhibits human brain microvascular endothelial cell adhesion, migration, proliferation, and tubulogenesis. To evaluate the inhibitory effect of LCT on ocular angiogenesis, we cultured aortic and choroidal explants in the presence of LCT and analyzed the effect of LCT on CNV in the mouse CNV model and on retinal neovascularization in the oxygen\u2010induced retinopathy model. Our data demonstrate that a single injection of LCT efficiently reduced CNV and retinal neovascularization in these models.\u2014Montassar, F., Darche, M., Blaizot, A., Augustin, S., Conart, J.\u2010B., Millet, A., Elayeb, M., Sahel, J.\u2010A., R\u00e9aux\u2010Le Goazigo, A., Sennlaub, F., Marrakchi, N., Messadi, E., Guillonneau, X. Lebecetin, a C\u2010type lectin, inhibits choroidal and retinal neovascularization. FASEB J. 31, 1107\u20131119 (2017).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201600351r","type":"journal-article","created":{"date-parts":[[2016,12,14]],"date-time":"2016-12-14T22:50:14Z","timestamp":1481755814000},"page":"1107-1119","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Lebecetin, a C\u2010type lectin, inhibits choroidal and retinal neovascularization"],"prefix":"10.1096","volume":"31","author":[{"given":"Fadoua","family":"Montassar","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9s Universit\u00e9 Pierre et Marie Curie INSERM Centre National de la Recherche Scientifique Institut de la Vision  Paris France"},{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques LR11IPT08 Institut Pasteur de Tunis  Tunis Tunisia"},{"name":"Universit\u00e9 de Carthage Facult\u00e9 des Sciences de Bizerte  Bizerte Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Darche","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s Universit\u00e9 Pierre et Marie Curie INSERM Centre National de la Recherche Scientifique Institut de la Vision  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Blaizot","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s Universit\u00e9 Pierre et Marie Curie INSERM Centre National de la Recherche Scientifique Institut de la Vision  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Augustin","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s Universit\u00e9 Pierre et Marie Curie INSERM Centre National de la Recherche Scientifique Institut de la Vision  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Baptiste","family":"Conart","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s Universit\u00e9 Pierre et Marie Curie INSERM Centre National de la Recherche Scientifique Institut de la Vision  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lie","family":"Millet","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques LR11IPT08 Institut Pasteur de Tunis  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Elayeb","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques LR11IPT08 Institut Pasteur de Tunis  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9-Alain","family":"Sahel","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s Universit\u00e9 Pierre et Marie Curie INSERM Centre National de la Recherche Scientifique Institut de la Vision  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabelle R\u00e9aux-Le","family":"Goazigo","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s Universit\u00e9 Pierre et Marie Curie INSERM Centre National de la Recherche Scientifique Institut de la Vision  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Sennlaub","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s Universit\u00e9 Pierre et Marie Curie INSERM Centre National de la Recherche Scientifique Institut de la Vision  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naziha","family":"Marrakchi","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques LR11IPT08 Institut Pasteur de Tunis  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erij","family":"Messadi","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques LR11IPT08 Institut Pasteur de Tunis  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Guillonneau","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s Universit\u00e9 Pierre et Marie Curie INSERM Centre National de la Recherche Scientifique Institut de la Vision  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.122.4.564"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.122.4.552"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2003.11.069"},{"key":"e_1_2_8_5_1","first-page":"3974","article-title":"Mechanisms of retinal and choroidal neovascularization","volume":"35","author":"D'Amore P. A.","year":"1994","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa062655"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa054481"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0b013e3181a2c1c3"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2011.203893"},{"key":"e_1_2_8_10_1","first-page":"CD008721","article-title":"Anti-vascular endothelial growth factor for proliferative diabetic retinopathy","volume":"11","author":"Martinez-Zapata M. J.","year":"2014","journal-title":"Cochrane Database Syst. Rev."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2014.10.021"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90007-8"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5241.1500"},{"key":"e_1_2_8_14_1","first-page":"3475","article-title":"Immunolocalization of integrins in proliferative retinal membranes","volume":"35","author":"Robbins S. G.","year":"1994","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9764"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2012.04.002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.105.020941"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-0469"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2009.265"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/s00417-008-1010-5","article-title":"Anti-angiogenic effects of non-peptide integrin alphavbeta3 specific antagonist on laser-induced choroidal neovascularization in mice","volume":"247","author":"Honda S.","year":"2009","journal-title":"Albrecht Von Graefes Arch. Klin. Exp. Ophthalmol."},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018864"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","first-page":"7213","DOI":"10.1167\/iovs.10-7097","article-title":"The antiangiogenic effects of integrin alpha5beta1 inhibitor (ATN-161) in vitro and in vivo","volume":"52","author":"Wang W.","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-9639(03)00232-2"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2007.01.001"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20935"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.01.013"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069552"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132644"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096494"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.187"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6989(85)90076-8"},{"key":"e_1_2_8_33_1","first-page":"864","article-title":"Mechanism for laser-induced neovascularization in rat choroid: accumulation of integrin \u03b1 chain-positive cells and their ligands","volume":"20","author":"Nakajima T.","year":"2014","journal-title":"Mol. Vis."},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2015.12.017"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1999.0814"},{"key":"e_1_2_8_36_1","doi-asserted-by":"crossref","first-page":"786870","DOI":"10.1155\/2012\/786870","article-title":"Anti-VEGF treatment strategies for wet AMD","volume":"2012","author":"Kovach J. L.","year":"2012","journal-title":"J. Ophthalmol."},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-011-9249-6"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.135"},{"key":"e_1_2_8_39_1","first-page":"101","article-title":"Oxygen-induced retinopathy in the mouse","volume":"35","author":"Smith L. E.","year":"1994","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-009-9155-3"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-13397"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/10623320600698037"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0841fje"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1941"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0214268"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65005-5"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81618-9"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2009.09.014"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-8-173"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0207114"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00021.2004"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021411"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-5155(02)00482-3"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-001-0727-z"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-1314"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.107.131656"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201600351R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201600351R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201600351R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T19:39:49Z","timestamp":1761680389000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201600351R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,14]]},"references-count":55,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1096\/fj.201600351R"],"URL":"https:\/\/doi.org\/10.1096\/fj.201600351r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,14]]},"assertion":[{"value":"2016-05-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-12-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}