{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:35:45Z","timestamp":1762176945301,"version":"build-2065373602"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,9,13]],"date-time":"2016-09-13T00:00:00Z","timestamp":1473724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007195","name":"Universit\u00e0 degli Studi di Napoli Federico II","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007195","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The developmental role of the endocannabinoid system still remains to be fully understood. Here, we report thepresenceof a complete endocannabinoid system during zebrafish development andshowthat thegenes that code for enzymes that catalyze the anabolism and catabolism (\n                    <jats:italic>mgll<\/jats:italic>\n                    and\n                    <jats:italic>dagla<\/jats:italic>\n                    ) of the endocannabinoid, 2\u2010AG (2\u2010arachidonoylglycerol), as well as 2\u2010AGmain receptor in the brain, cannabinoid receptor type 1, are coexpressed in defined regions of axonal growth. By using morpholino\u2010induced transient knockdown of the zebrafish\n                    <jats:italic>Dagl\u03b1<\/jats:italic>\n                    homologandits pharmacologic rescue, we suggest that synthesis of 2\u2010AGis implicated inthe control of axon formationin themidbrain\u2010hindbrain region andthat animals that lack\n                    <jats:italic>Dagl<\/jats:italic>\n                    \u03b1 display abnormal physiological behaviors intests that measure stereotypedmovement andmotion perception. Our results suggest that thewell\u2010established role for 2\u2010AG in axonal outgrowth has implications for the control of vision andmovement in zebrafish and, thus, is likely common to all vertebrates.\u2014Martella, A., Sepe, R.M., Silvestri, C., Zang, J., Fasano, G., Carnevali, O., De Girolamo, P., Neuhauss, S.C. F., Sordino, P., Di Marzo, V. Important role of endocannabinoid signaling inthe development of functional vision and locomotion in zebrafish. FASEB J. 30, 4275\u20134288 (2016).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201600602r","type":"journal-article","created":{"date-parts":[[2016,9,13]],"date-time":"2016-09-13T22:09:23Z","timestamp":1473804563000},"page":"4275-4288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Important role of endocannabinoid signaling in the development of functional vision and locomotion in zebrafish"],"prefix":"10.1096","volume":"30","author":[{"given":"Andrea","family":"Martella","sequence":"first","affiliation":[{"name":"Endocannabinoid Research Group Institute of Biomolecular Chemistry Consiglio Nazionale delle Ricerche  Pozzuoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa M.","family":"Sepe","sequence":"additional","affiliation":[{"name":"Biology and Evolution of Marine Organisms Stazione Zoologica Anton Dohrn  Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristoforo","family":"Silvestri","sequence":"additional","affiliation":[{"name":"Endocannabinoid Research Group Institute of Biomolecular Chemistry Consiglio Nazionale delle Ricerche  Pozzuoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Zang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Life Sciences University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Fasano","sequence":"additional","affiliation":[{"name":"Biology and Evolution of Marine Organisms Stazione Zoologica Anton Dohrn  Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliana","family":"Carnevali","sequence":"additional","affiliation":[{"name":"\u00a7Department of Life and Environment Sciences Polytechnic University of Marche  Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"De Girolamo","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Produzioni Animali Universtity of Naples Federico II  Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan C. F.","family":"Neuhauss","sequence":"additional","affiliation":[{"name":"Institute of Molecular Life Sciences University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Sordino","sequence":"additional","affiliation":[{"name":"Biology and Evolution of Marine Organisms Stazione Zoologica Anton Dohrn  Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzo","family":"Di Marzo","sequence":"additional","affiliation":[{"name":"Endocannabinoid Research Group Institute of Biomolecular Chemistry Consiglio Nazionale delle Ricerche  Pozzuoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705666"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2010.03.007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200305129"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/372686a0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/384083a0"},{"key":"e_1_2_9_7_1","article-title":"Synthesis of prostaglandin E2 ethanolamide from anandamide by cyclooxygenase\u20102","volume":"272","author":"Yu M.","year":"1997","journal-title":"J. Biol. Chem"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M007088200","article-title":"Oxygenation of the endocannabinoid, 2\u2010arachidonylglycerol, to glyceryl prostaglandins by cyclooxygenase\u20102","volume":"275","author":"Kozak K. R.","year":"2000","journal-title":"J. Biol. Chem"},{"key":"e_1_2_9_9_1","article-title":"Brain monoglyceride lipase participating in endocannabinoid inactivation","volume":"99","author":"Dinh T. P.","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1470919"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2437"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2007.01.005"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prostaglandins.2009.07.001"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2396(19990901)33:3<181::AID-SYN3>3.0.CO;2-R"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(03)00020-4"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803545105"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137406"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2008.02.001"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2011.0394"},{"volume-title":"The Cannabinoid Receptors (Reggio, P. H., ed.), pp. 123\u2013149","year":"2009","author":"Elphick M. R.","key":"e_1_2_9_20_1"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.10.069"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20502"},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","first-page":"4421","DOI":"10.1038\/ncomms5421","article-title":"Endocannabinoidsmodulatecortical development by configuring Slit2\/Robo1 signalling","volume":"5","author":"Alp\u00e1ar A.","year":"2014","journal-title":"Nat. Commun"},{"volume-title":"A Guide for the Laboratory Use of Zebrafish (Danio rerio), 4th ed","year":"2000","author":"Westerfield M.","key":"e_1_2_9_24_1"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2007.09.002"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707424"},{"key":"e_1_2_9_28_1","first-page":"1229","article-title":"Zebrafish whole mount high\u2010resolution double fluorescent in situ hybridization","volume":"25","author":"Brend T.","year":"2009","journal-title":"J. Vis. Exp"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOL.0b013e32803dbdec"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-007-0207-3"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells3030914"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2011.00001"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061029898"},{"key":"e_1_2_9_34_1","doi-asserted-by":"crossref","DOI":"10.1523\/JNEUROSCI.2126-10.2010","article-title":"Differential subcellular recruitment of monoacylglycerol lipase generates spatial specificity of 2\u2010arachidonoyl glycerol signaling during axonal pathfinding","volume":"30","author":"Keimpema E.","year":"2010","journal-title":"J. Neurosci"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-12514"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-9994-7-8"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00007.2010"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphysparis.2003.10.009"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.11.004"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2760(97)00009-X"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2004.05.002"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bjh009"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(01)00953-0"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0803274"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2008.11.014"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8104-8-3"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2011.0382"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1212563110"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00044-3"},{"key":"e_1_2_9_50_1","doi-asserted-by":"crossref","first-page":"4513","DOI":"10.1523\/JNEUROSCI.08-12-04513.1988","article-title":"Retinotopic organization of the developing retinotectal projection in the zebrafish embryo","volume":"8","author":"Stuermer C. A.","year":"1988","journal-title":"J. Neurosci"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2678-05.2005"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.03.024"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2013.00053"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03406.x"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5850-11.2012"},{"key":"e_1_2_9_56_1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0022-3565(24)29527-6","article-title":"Zebra finch CB1 cannabinoid receptor: pharmacology and in vivo and in vitro effects of activation","volume":"297","author":"Soderstrom K.","year":"2001","journal-title":"J. Pharmacol. Exp. Ther"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053082"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200210164"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.110.070458"},{"key":"e_1_2_9_60_1","doi-asserted-by":"crossref","DOI":"10.1523\/JNEUROSCI.3174-07.2007","article-title":"Long\u2010term plasticity of the spinal locomotor circuitry mediated by endocannabinoid and nitric oxide signaling","volume":"27","author":"Kyriakatos A.","year":"2007","journal-title":"J. Neurosci"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201600602R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201600602R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201600602R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:53:03Z","timestamp":1761691983000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201600602R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,13]]},"references-count":59,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1096\/fj.201600602R"],"URL":"https:\/\/doi.org\/10.1096\/fj.201600602r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"type":"print","value":"0892-6638"},{"type":"electronic","value":"1530-6860"}],"subject":[],"published":{"date-parts":[[2016,9,13]]},"assertion":[{"value":"2016-05-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}