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Here we report that suppressing of 15\u2010lipoxygenase\u20101 led to a substantial reduction in DC spreading and podosome formation in vitro. The surface expression of CD83 was significantly lower in both sh\u201015\u2010lipoxygenase\u20101 (15\u2010LOX\u20101)\u2010transduced cells and DCs cultivated in the presence of a novel specific 15\u2010LOX\u20101 inhibitor. The T\u2010cell response against tetanus\u2010pulsed DCs was only affected to a minor extent on inhibition of 15\u2010LOX\u20101. In contrast, endocytosis and migration ability of DCs were significantly suppressed on 15\u2010LOX\u20101 inhibition. The expression of 15\u2010LOX\u20101 in DCs was also demonstrated in affected human skin in atopic and contact dermatitis, showing that the enzyme is indeed expressed in inflammatory diseases in vivo. This study demonstrated that inhibiting 15\u2010LOX\u20101 ledtoanimpairedpodosome formation in DCs, and consequently suppressed antigen uptake and migration capacity. These results indicated that 15\u2010LOX\u20101 is a potential target for inhibiting the trafficking of DCs to lymphoid organs and inflamed tissues and decreasing the inflammatory response attenuating symptoms of certain immunologic and inflammatory disorders such as dermatitis.\u2014Han, H., Liang, X., Ekberg, M., Kritikou, J. S., Brunnstr\u00f6m, \u00c5., Pelcman, B., Matl, M., Miao, X., Andersson, M., Yuan, X., Schain, F., Parvin, S., Melin, E., Sj\u00e5berg, J., Xu, D., Westerberg, L. S., Bj\u00e5rkholm, M., Claesson, H.\u2010E. Human 15\u2010lipoxygenase\u20101 is a regulator of dendritic\u2010cell spreading and podosome formation. FASEB J. 31, 491\u2013504 (2017).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201600679rr","type":"journal-article","created":{"date-parts":[[2016,10,18]],"date-time":"2016-10-18T16:40:11Z","timestamp":1476808811000},"page":"491-504","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Human 15\u2010lipoxygenase\u20101 is a regulator of dendriticcell spreading and podosome formation"],"prefix":"10.1096","volume":"31","author":[{"given":"Hongya","family":"Han","sequence":"first","affiliation":[{"name":"Department of Medicine, Center for Hematology and Regenerative Medicine, Karolinska Institutet Karolinska University Hospital Huddinge Stockholm Sweden"},{"name":"Division of Hematology Karolinska University Hospital Solna Stockholm 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