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Here, we confirmed KCNE3 transcript and protein expression in mouse skeletal muscle using\n                    <jats:italic>\n                      Kcne3\n                      <jats:sup>\u2010\/\u2010<\/jats:sup>\n                    <\/jats:italic>\n                    tissue as a negative control. Whole\u2010transcript microarray analysis (770,317 probes, interrogating 28,853 transcripts) findings were consistent with\n                    <jats:italic>Kcne3<\/jats:italic>\n                    deletion increasing gastrocnemius oxidative metabolic gene expression and the proportion of type IIa fast\u2010twitch oxidative muscle fibers, which was verified using immunofluorescence. The down\u2010regulated transcript set overlapped with muscle unloading gene expression profiles (\u20211.5\u2010fold change; P &lt; 0.05). Gastrocnemius K\n                    <jats:sup>+<\/jats:sup>\n                    channel a subunit remodeling arising from\n                    <jats:italic>Kcne3<\/jats:italic>\n                    deletion was highly specific, involving just 3 of 69 a subunit genes probed: known KCNE3 partners KCNC4 and KCNH2 (mERG) were down\u2010regulated, and KCNK4 (TRAAK) was up\u2010regulated (P &lt; 0.05). Functionally,\n                    <jats:italic>\n                      Kcne3\n                      <jats:sup>\u2010\/\u2010<\/jats:sup>\n                    <\/jats:italic>\n                    mice exhibited abnormal hind\u2010limb clasping upon tail suspension (63% of\n                    <jats:italic>\n                      Kcne3\n                      <jats:sup>\u2010\/\u2010<\/jats:sup>\n                    <\/jats:italic>\n                    mice \u202110\u2010mo\u2010old vs. 0% age\u2010matched\n                    <jats:italic>\n                      Kcne3\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    littermates). Whereas 5 of 5\n                    <jats:italic>\n                      Kcne3\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    mice exhibited the typical biphasic decline in contractile force with repetitive stimuli of hind\u2010limb muscle, both\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    , this was absent in 6 of 6\n                    <jats:italic>\n                      Kcne3\n                      <jats:sup>\u2010\/\u2010<\/jats:sup>\n                    <\/jats:italic>\n                    mice tested. Finally, myoblasts isolated from\n                    <jats:italic>\n                      Kcne3\n                      <jats:sup>\u2010\/\u2010<\/jats:sup>\n                    <\/jats:italic>\n                    mice exhibit faster\u2010inactivating and smaller sustained outward currents than those from\n                    <jats:italic>\n                      Kcne3\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    mice. Thus,\n                    <jats:italic>Kcne3<\/jats:italic>\n                    deletion impairs skeletal muscle function in mice.\u2014King, E. C., Patel, V., Anand, M., Zhao, X., Crump, S. M., Hu, Z., Weisleder, N., Abbott, G. W. Targeted deletion of\n                    <jats:italic>Kcne3<\/jats:italic>\n                    impairs skeletal muscle function in mice. FASEB J. 31, 2937\u20132947 (2017).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201600965rr","type":"journal-article","created":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T20:50:29Z","timestamp":1490820629000},"page":"2937-2947","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Targeted deletion of Kcne3 impairs skeletal muscle function in mice"],"prefix":"10.1096","volume":"31","author":[{"given":"Elizabeth C.","family":"King","sequence":"first","affiliation":[{"name":"Department of Pharmacology Weill Medical College of Cornell University New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishal","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics University of Medicine and Dentistry of New Jersey\u2013Robert Wood Johnson Medical School Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Anand","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine University of California Irvine California USA"},{"name":"Department of Physiology and Biophysics School of Medicine University of California Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics University of Medicine and Dentistry of New Jersey\u2013Robert Wood Johnson Medical School Piscataway New Jersey USA"},{"name":"Department of Physiological Sciences Eastern Virginia Medical School Norfolk VA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn M.","family":"Crump","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine University of California Irvine California USA"},{"name":"Department of Physiology and Biophysics School of Medicine University of California Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine University of California Irvine California USA"},{"name":"Department of Physiology and Biophysics School of Medicine University of California Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah","family":"Weisleder","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics University of Medicine and Dentistry of New Jersey\u2013Robert Wood Johnson Medical School Piscataway New Jersey USA"},{"name":"Department of Physiology and Cell Biology Davis Heart and Lung Research Institute Ohio State University Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey W.","family":"Abbott","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine University of California Irvine California USA"},{"name":"Department of Physiology and Biophysics School of Medicine University of California Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80728-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/35003200"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00207-0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000082392.66713.E3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000119392.29624.88"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.047829"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-173682"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2007.01.009"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.06.050"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/19.2.185"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-1904-8"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2307\/3001534"},{"key":"e_1_2_6_14_1","volume-title":"Statistics and Data Analysis from Elementary to Intermediate","author":"Tamhane A. 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