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EP4 knockdown using gene\u2010specific small interfering RNA did not affect the rapid phase of OxPAPC\u2010induced EC barrier enhancement or the protective effects against thrombin\u2010induced EC permeability, but abolished the advanced barrier enhancement phase and suppressed the protective effects of OxPAPC against more sustained EC barrier dysfunction and cell inflammatory response caused by TNF\u2010\u03b1. Endothelial\u2010specific knockout of the EP4 receptor in mice attenuated the protective effect of intravenous OxPAPC administration in the model of acute lung injury caused by intratracheal injection of LPS. Taken together, these results demonstrate a novel role for prostaglandin receptor EP4 in the mediation of barrier\u2010enhancing and anti\u2010inflammatory effects caused by oxidized phospholipids.\u2014Oskolkova, O., Gawlak, G., Tian, Y., Ke, Y., Sarich, N., Son, S., Andreasson, K., Bochkov, V. N., Birukova, A. A., Birukov, K. G. Prostaglandin E receptor\u20104 receptor mediates endothelial barrier\u2013enhancing and anti\u2010inflammatory effects of oxidized phospholipids. FASEB J. 31, 4187\u20134202 (2017).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                    \u2014Branco, Renato Chaves Souto, Camargo, Rafael Ludemann, Batista, Thiago Martins, Vettorazzi, Jean Franciesco, Borck, Patricia Cristine, dos Santos\u2010Silva, Junia Carolina Rebelo, Boschero, Antonio Carlos, Zoppi, Claudio Cesar, Carneiro, Everardo Magalh\u00e4es Prostaglandin E receptor\u20104 receptor mediates endothelial barrier\u2013enhancing and anti\u2010inflammatory effects of oxidized phospholipids. 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