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Here, we pretreated MLO\u2010Y4 osteocytes with 10 nM E\n                    <jats:sub>2<\/jats:sub>\n                    for 2, 3 and 5 d, then simulated postmenopausal conditions either by estrogen withdrawal (EW) from culture medium, or by inhibiting the estrogen receptor by using fulvestrant and estrogen (FE; ICI 182,780)\n                    <jats:italic>in vitro<\/jats:italic>\n                    . We investigated [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    oscillations and mechanobiologic responses of osteocytes (EW and FE) that were exposed to oscillatory fluid flow (OFF; 1 Pa, 0.5 Hz). We demonstrated that estrogen treatment enhanced OFF\u2010induced [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    oscillations and that this effect was abrogated both by FE and EW. Moreover, osteocytes in both estrogen\u2010depleted groups (EW and FE) had reduced levels of NO and prostaglandin E\n                    <jats:sub>2<\/jats:sub>\n                    release, down\u2010regulated dentin matrix protein\u20101, sclerostin, osteopontin, osteocalcin, and alkaline phosphatase mRNA expression, and reduced F\u2010actin fiber formation after OFF stimulation compared with estrogen\u2010treated cells. We propose a link between estrogen deficiency and alterations in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    \u2010mediated mechanosensitivity of osteocytes, which ultimately alter osteocyte function and differentiation.\u2014Deepak, V., Kayastha, P., McNamara, L. M. Estrogen deficiency attenuates fluid flow\u2013induced [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    oscillations and mechanoresponsiveness of MLO\u2010Y4 osteocytes. FASEB J. 31, 3027\u20133039 (2017).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201601280r","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T21:00:20Z","timestamp":1490994020000},"page":"3027-3039","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Estrogen deficiency attenuates fluid flow\u2010induced [Ca\n                    <sub>2+<\/sub>\n                    ]\n                    <sub>i<\/sub>\n                    oscillations and mechanoresponsiveness of MLO\u2010Y4 osteocytes"],"prefix":"10.1096","volume":"31","author":[{"given":"Vishwa","family":"Deepak","sequence":"first","affiliation":[{"name":"Mechanobiology and Medical Device Research Group Biomechanics Research Centre Biomedical Engineering College of Engineering and Informatics National University of Ireland Galway Galway Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pushpalata","family":"Kayastha","sequence":"additional","affiliation":[{"name":"Mechanobiology and Medical Device Research Group Biomechanics Research Centre Biomedical Engineering College of Engineering and Informatics National University of Ireland Galway Galway Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laoise M.","family":"McNamara","sequence":"additional","affiliation":[{"name":"Mechanobiology and Medical Device Research Group Biomechanics Research Centre Biomedical Engineering College of Engineering and Informatics National University of Ireland Galway Galway Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-198466030-00012"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2014.06.017"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20603"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2012.10.013"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650110918"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-012-0105-4"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.5.7896017"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-10-0912"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2015.03.019"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407429101"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707246104"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2012.0286"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10237-013-0487-y"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v023a02"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.05.001"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.2.249"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700636104"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20869"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-006-0716-x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2014.11.001"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2822"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.02.175"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1474"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1996.270.4.E634"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.6.963"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1155"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1999.276.1.E171"},{"key":"e_1_2_9_29_1","first-page":"143","article-title":"Oestrogen action on bone cells","volume":"2","author":"Krassas G. 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