{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:35:45Z","timestamp":1787009745745,"version":"build-2736575974"},"reference-count":74,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T00:00:00Z","timestamp":1500249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["U01-ES019545"],"award-info":[{"award-number":["U01-ES019545"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["U01-ES0200126"],"award-info":[{"award-number":["U01-ES0200126"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["T32-ES015459"],"award-info":[{"award-number":["T32-ES015459"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["P30-ES007033"],"award-info":[{"award-number":["P30-ES007033"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["P30-ES000260"],"award-info":[{"award-number":["P30-ES000260"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Silver nanoparticles (AgNPs) are employed in a variety of consumer products; however,\n                    <jats:italic>in vivo<\/jats:italic>\n                    rodent studies indicate that AgNPs can cause lung inflammation and toxicity in a strain\u2010 and particle type\u2013dependent manner, but mechanisms of susceptibility remain unclear. The aim of this study was to assess the variation in AgNP\u2010induced lung inflammation and toxicity across multiple inbred mouse strains and to use genome\u2010wide association (GWA) mapping to identify potential candidate susceptibility genes. Mice received doses of 0.25 mg\/kg of either 20\u2010nm citrate\u2010coated AgNPs or citrate buffer using oropharyngeal aspiration. Neutrophils in bronchoalveolar lavage fluid (BALF) served as markers of inflammation. We found significant strain\u2010 and treatment\u2010dependent variation in neutrophils in BALF. GWA mapping identified 10 significant single\u2010nucleotide polymorphisms (false discovery rate, 15%) in 4 quantitative trait loci on mouse chromosomes 1, 4, 15, and 18, and\n                    <jats:italic>Nedd4l<\/jats:italic>\n                    (neural precursor cell expressed developmentally downregulated gene 4\u2010like; chromosome 18),\n                    <jats:italic>Ano6<\/jats:italic>\n                    (anocatmin 6; chromosome 15), and\n                    <jats:italic>Rnf220<\/jats:italic>\n                    (Ring finger protein 220; chromosome 4) were considered candidate genes. Quantitative RT\u2010PCR revealed significant inverse associations between mRNA levels of these genes and neutrophil influx.\n                    <jats:italic>Nedd4l, Ano6<\/jats:italic>\n                    , and\n                    <jats:italic>Rnf220<\/jats:italic>\n                    are candidate susceptibility genes for AgNP\u2010induced lung inflammation that warrant additional exploration in future studies.\u2014Scoville, D. K., Botta, D., Galdanes, K., Schmuck, S. C., White, C. C., Stapleton, P. L., Bammler, T. K., MacDonald, J. W., Altemeier, W. A., Hernandez, M., Kleeberger, S. R., Chen, L.\u2010C., Gordon, T., Kavanagh, T. J. Genetic determinants of susceptibility to silver nanoparticle\u2010induced acute lung inflammation in mice. FASEB J. 31, 4600\u20134611 (2017).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700187r","type":"journal-article","created":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T21:05:26Z","timestamp":1500325526000},"page":"4600-4611","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Genetic determinants of susceptibility to silver nanoparticle\u2010induced acute lung inflammation in mice"],"prefix":"10.1096","volume":"31","author":[{"given":"David K.","family":"Scoville","sequence":"first","affiliation":[{"name":"Department of Environmental and Occupational Health Sciences University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianne","family":"Botta","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health Sciences University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Galdanes","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine New York University Tuxedo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie C.","family":"Schmuck","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health Sciences University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Collin C.","family":"White","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health Sciences University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia L.","family":"Stapleton","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health Sciences University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo K.","family":"Bammler","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health Sciences University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"MacDonald","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health Sciences University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William A.","family":"Altemeier","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Hernandez","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine New York University Tuxedo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven R.","family":"Kleeberger","sequence":"additional","affiliation":[{"name":"Immunity, Inflammation, and Disease Laboratory National Institute of Environmental Health Sciences Research Triangle Park North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lung\u2010Chi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine New York University Tuxedo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry","family":"Gordon","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine New York University Tuxedo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terrance J.","family":"Kavanagh","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine New York University Tuxedo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,17]]},"reference":[{"key":"e_1_2_9_2_1","author":"The Project on Emerging Nanotechnologies","journal-title":"Consumer products inventory"},{"key":"e_1_2_9_3_1","volume-title":"Exposure assessment and inflammatory response among workers producing calcium carbonate nanomaterials","author":"Cui L.","year":"2013"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2014.978404"},{"issue":"1","key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"100","DOI":"10.3109\/17435390.2013.858793","article-title":"Six\u2010month follow\u2010up study of health markers of nanomaterials among workers handling engineered nanomaterials","volume":"8","author":"Liao H.\u2010Y.","year":"2014","journal-title":"Nanotoxicology"},{"key":"e_1_2_9_6_1","author":"The National Institute for Occupational Safety and Health","year":"1994","journal-title":"Immediately dangerous to life or health"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2016.1148793"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.02.021"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-11-11"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2010.483745"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201301597"},{"key":"e_1_2_9_12_1","first-page":"129","article-title":"Demonstrating approaches to chemically modify the surface of Ag nanoparticles in order to influence their cytotoxicity and biodistribution after single dose acute intravenous administration","volume":"10","author":"Pang C.","year":"2016","journal-title":"Nanotoxicology"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119726"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3399"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112743"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.178"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3561"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfv005"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2014.958116"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfu265"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623316629804"},{"key":"e_1_2_9_22_1","first-page":"118","article-title":"Low\u2010dose AgNPs reduce lung mechanical function and innate immune defense in the absence of cellular toxicity","volume":"10","author":"Botelho D. J.","year":"2016","journal-title":"Nanotoxicology"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.26.5.4737"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.090241.108"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfu094"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1259595"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.114.168138"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2014.00272"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-247221"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.118786.110"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.12.007"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-002-2187-2"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074001"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg412"},{"key":"e_1_2_9_35_1","author":"Pinheiro J.","journal-title":"R Core Team. nlme: linear and nonlinear mixed effects models"},{"key":"e_1_2_9_36_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1101\/pdb.top77","article-title":"Variance component methods for analysis of complex phenotypes","volume":"2010","author":"Almasy L.","year":"2010","journal-title":"Cold Spring Harb. Protoc."},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.112.140640"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2322"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.080101"},{"key":"e_1_2_9_40_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/B978-0-12-179060-8.50007-4","volume-title":"Statistical Power Analysis for the Behavioral Sciences, pp.","author":"Cohen J.","year":"1977"},{"key":"e_1_2_9_41_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/s12863-016-0411-4","article-title":"Consomic mouse strain selection based on effect size measurement, statistical significance testing and integrated behavioral z\u2010scoring: focus on anxiety\u2010related behavior and locomotion","volume":"17","author":"Labots M.","year":"2016","journal-title":"BMC Genet."},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.229102"},{"key":"e_1_2_9_43_1","author":"Turner S. D.","year":"2014","journal-title":"qqman: an R package for visualizing GWAS results using Q\u2010Q and manhattan plots"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-98141-3"},{"key":"e_1_2_9_45_1","volume-title":"R: A Language and Environment for Statistical Computing,","author":"R Core Team","year":"2014"},{"key":"e_1_2_9_46_1","volume-title":"Lattice: Multivariate Data Visualization With R,","author":"Deepayan S.","year":"2008"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2015.09.019"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064165"},{"key":"e_1_2_9_49_1","author":"The Jackson Laboratory","year":"2011","journal-title":"Stearns1: Lung Gene Expression in Females of 9 Inbred Strains of Mice. MPD: Stearns1"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2825804"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.111310.110"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.039313"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.138032"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/55.1.24"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1080\/01902140600817465"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.6b05652"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0191com"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1028"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010334108"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2013.96"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7245"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00039.2011"},{"key":"e_1_2_9_63_1","first-page":"127","article-title":"Beyond apoptosis: the mechanism and function of phosphatidylserine asymmetry in the membrane of activating mast cells","volume":"4","author":"Rysavy N. M.","year":"2014","journal-title":"BioArchitecture"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.06901"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.4.1409.bloodjournal8741409"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09583"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.07.036"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2012.08.020"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2011.00057"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2010.81"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108094108"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00731-14"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-02-622233"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1539"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200318921"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201700187R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201700187R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201700187R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:50:08Z","timestamp":1761673808000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201700187R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,17]]},"references-count":74,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1096\/fj.201700187R"],"URL":"https:\/\/doi.org\/10.1096\/fj.201700187r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,17]]},"assertion":[{"value":"2017-03-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-06-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}