{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T05:04:14Z","timestamp":1775797454054,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,5]],"date-time":"2018-01-05T00:00:00Z","timestamp":1515110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1U54HD090257\u201001"],"award-info":[{"award-number":["1U54HD090257\u201001"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>\n                    Nontypeable\n                    <jats:italic>Haemophilus influenzae<\/jats:italic>\n                    (NTHi), one of the most common acute otitis media (OM) pathogens, is postulated to promote middle\u2010ear epithelial remodeling in the progression of OM from acute to chronic. The goal of this study was to examine early quantitative proteomic secretome effects of NTHi lysate exposure in a human middle\u2010ear epithelial cell (HMEEC) line. NTHi lysates were used to stimulate HMEEC, and conditional quantitative stable isotope labeling with amino acids in cell culture of cell secretions was performed. Mass spectrometry analysis identified 766 proteins across samples. Of interest, several heterogeneous nuclear ribonucleoproteins (hnRNPs) were regulated by NTHi lysate treatment, especially hnRNP A2B1 and hnRNP Q, known to be implicated in microRNA (miRNA) packaging in exosomes. After purification, the presence of exosomes in HMEEC secretions was characterized by dynamic light scattering (&lt;100 nm), transmission electron microscopy, and CD63\/heat shock protein 70 positivity. hnRNP A2B1 and hnRNP Q were confirmed to be found in exosomes by Western blot and proteomic analysis. Finally, exosomal miRNA content comprised 110 unique miRNAs, with 5 found to be statistically induced by NTHi lysate (miR\u2010378a\u20103p+miR\u2010378i, miR\u2010200a\u20103p, miR\u2010378g, miR30d\u20105p, and miR\u2010222\u20103p), all known to target innate immunity genes. This study demonstrates that NTHi lysates promote release of miRNAladen exosomes from middle\u2010ear epithelium\n                    <jats:italic>in vitro<\/jats:italic>\n                    .\u2014 Val, S., Krueger, A., Poley, M., Cohen, A., Brown, K., Panigrahi, A., Preciado, D. Nontypeable\n                    <jats:italic>Haemophilus influenzae<\/jats:italic>\n                    lysates increase heterogeneous nuclear ribonucleoprotein secretion and exosome release in human middle\u2010ear epithelial cells.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    32, 1855\u20131867 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700248rr","type":"journal-article","created":{"date-parts":[[2017,11,30]],"date-time":"2017-11-30T12:05:23Z","timestamp":1512043523000},"page":"1855-1867","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Nontypeable\n                    <i>Haemophilus influenzae<\/i>\n                    lysates increase heterogeneous nuclear ribonucleoprotein secretion and exosome release in human middle\u2010ear epithelial cells"],"prefix":"10.1096","volume":"32","author":[{"given":"Stephanie","family":"Val","sequence":"first","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Krueger","sequence":"additional","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marian","family":"Poley","sequence":"additional","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariella","family":"Cohen","sequence":"additional","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristy","family":"Brown","sequence":"additional","affiliation":[{"name":"Center for Genetic Medicine Research Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aswini","family":"Panigrahi","sequence":"additional","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Preciado","sequence":"additional","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation Washington DC USA"},{"name":"Division of Pediatric Otolaryngology Children's National Health System Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0009922810370363"},{"key":"e_1_2_9_3_1","first-page":"1","article-title":"National Ambulatory Medical Care Survey: 2000 summary","author":"Cherry D. 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