{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T03:08:40Z","timestamp":1779073720362,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T00:00:00Z","timestamp":1505779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Deficiency of the cysteine protease inhibitor cystatin M\/E (Cst6) in mice leads to disturbed epidermal cornification, impaired barrier function, and neonatal lethality. We report the rescue of the lethal skin phenotype of\n                    <jats:italic>ichq<\/jats:italic>\n                    (Cst6\u2010deficient;\n                    <jats:italic>Cst6<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) mice by transgenic, epidermis\u2010specific, reexpression of Cst6 under control of the human involucrin (INV) promoter. Rescued Tg(INV\u2010\n                    <jats:italic>Cst6<\/jats:italic>\n                    )\n                    <jats:italic>Cst6<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>ichq\/ichq<\/jats:italic>\n                    <\/jats:sup>\n                    mice survive the neonatal phase, but display severe eye pathology and alopecia after 4 mo. We observed keratitis and squamous metaplasia of the corneal epithelium, comparable to\n                    <jats:italic>Cst6<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <jats:italic>Ctsl<\/jats:italic>\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    mice, as we have reported in other studies. We found the INV promoter to be active in the hair follicle infundibulum; however, we did not observe Cst6 protein expression in the lower regions of the hair follicle in Tg(INV\u2010\n                    <jats:italic>Cst6<\/jats:italic>\n                    )\n                    <jats:italic>Cst6<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>ichq\/ichq<\/jats:italic>\n                    <\/jats:sup>\n                    mice. This result suggests that unrestricted activity of proteases is involved in disturbance of hair follicle biology, eventually leading to baldness. Using quenched activity\u2010based probes, we identified mouse cathepsin B (CtsB), which is expressed in the lower regions of the hair follicle, as an additional target of mouse Cst6. These data suggest that Cst6 is necessary to control CtsB activity in hair follicle morphogenesis and highlight Cst6\u2010controlled proteolytic pathways as targets for preventing hair loss.\u2014Oortveld, M. A. W., van Vlijmen\u2010Willems, I. M. J. J., Kersten, F. F. J., Cheng, T., Verdoes, M., van Erp, P. E. J., Verbeek, S., Reinheckel, T., Hendriks, W. J. A. J., Schalkwijk, J., Zeeuwen, P. L. J. M. Cathepsin B as a potential cystatin M\/E target in the mouse hair follicle. FASEB J. 31, 4286\u20134294 (2017).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700267r","type":"journal-article","created":{"date-parts":[[2017,6,8]],"date-time":"2017-06-08T21:00:26Z","timestamp":1496955626000},"page":"4286-4294","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cathepsin B as a potential cystatin M\/E target in the mouse hair follicle"],"prefix":"10.1096","volume":"31","author":[{"given":"Merel A. W.","family":"Oortveld","sequence":"first","affiliation":[{"name":"Department of Dermatology Radboud University Nijmegen Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivonne M. J. J.","family":"van Vlijmen\u2010Willems","sequence":"additional","affiliation":[{"name":"Department of Dermatology Radboud University Nijmegen Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferry F. J.","family":"Kersten","sequence":"additional","affiliation":[{"name":"Department of Dermatology Radboud University Nijmegen Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsing","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Dermatology Radboud University Nijmegen Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martijn","family":"Verdoes","sequence":"additional","affiliation":[{"name":"Department of Tumor Immunology Radboud University Nijmegen Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piet E. 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