{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T21:23:56Z","timestamp":1781990636169,"version":"3.54.5"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T00:00:00Z","timestamp":1500854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["15411950600"],"award-info":[{"award-number":["15411950600"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:p>\n                    Osteoporosis is a metabolic bone disease characterized by decreased bone density and strength due to excessive loss of bone protein and mineral content. The imbalance between osteogenesis by osteoblasts and osteoclastogenesis by osteoclasts contributes to the pathogenesis of postmenopausal osteoporosis. Estrogen withdrawal leads to increased levels of proinflammatory cytokines. Overactivated osteoclasts by inflammation play a vital role in the imbalance. Matrine is an alkaloid found in plants from the\n                    <jats:italic>Sophora<\/jats:italic>\n                    genus with various pharmacological effects, including anti\u2010inflammatory activity. Here we demonstrate that matrine significantly prevented ovariectomy\u2010induced bone loss and inhibited osteoclastogenesis\n                    <jats:italic>in vivo<\/jats:italic>\n                    with decreased serum levels of TRAcp5b, TNF\u2010\u03b1, and IL\u20106.\n                    <jats:italic>In vitro<\/jats:italic>\n                    matrine significantly inhibited osteoclast differentiation induced by receptor activator for NF\u2010\u03baB ligand (RANKL) and M\u2010CSF in bone marrow monocytes and RAW264.7 cells as demonstrated by tartrate\u2010resistant acid phosphatase (TRAP) staining and actin\u2010ring formation as well as bone resorption through pit formation assays. For molecular mechanisms, matrine abrogated RANKL\u2010induced activation of NF\u2010\u03baB, AKT, and MAPK pathways and suppressed osteoclastogenesis\u2010related marker expression, including matrix metalloproteinase 9, NFATc1, TRAP, C\u2010Src, and cathepsin K. Our study demonstrates that matrine inhibits osteoclastogenesis through modulation of multiple pathways and that matrine is a promising agent in the treatment of osteoclast\u2010related diseases such as osteoporosis.\u2014Chen, X., Zhi, X., Pan, P., Cui, J., Cao, L., Weng, W., Zhou, Q., Wang, L., Zhai, X. Zhao, Q., Hu, H., Huang, B., Su, J. Matrine prevents bone loss in ovariectomized mice by inhibiting RANKL\u2010induced osteoclastogenesis. FASEB J. 31, 4855\u20134865 (2017).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700316r","type":"journal-article","created":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T20:50:21Z","timestamp":1500929421000},"page":"4855-4865","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["Matrine prevents bone loss in ovariectomized mice by inhibiting RANKL\u2010induced osteoclastogenesis"],"prefix":"10.1096","volume":"31","author":[{"given":"Xiao","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Orthopedics Trauma Second Military Medical University Shanghai China"},{"name":"China\u2010South Korea Bioengineering Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhi","sequence":"additional","affiliation":[{"name":"Graduate Management Unit Shanghai Changhai Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panpan","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Trauma Second Military Medical University Shanghai China"},{"name":"China\u2010South Korea Bioengineering Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Cui","sequence":"additional","affiliation":[{"name":"Graduate Management Unit Shanghai Changhai Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liehu","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Trauma Second Military Medical University Shanghai China"},{"name":"China\u2010South Korea Bioengineering Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weizong","family":"Weng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Trauma Second Military Medical University Shanghai China"},{"name":"China\u2010South Korea Bioengineering Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qirong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Trauma Second Military Medical University Shanghai China"},{"name":"China\u2010South Korea Bioengineering Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Trauma Second Military Medical University Shanghai China"},{"name":"China\u2010South Korea Bioengineering Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Trauma Second Military Medical University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingiie","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy Second Military Medical University Shanghai China"},{"name":"China\u2010South Korea Bioengineering Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honggang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Trauma Second Military Medical University Shanghai China"},{"name":"School of Pharmacy Second Military Medical University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biaotong","family":"Huang","sequence":"additional","affiliation":[{"name":"China\u2010South Korea Bioengineering Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiacan","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Trauma Second Military Medical University Shanghai China"},{"name":"China\u2010South Korea Bioengineering Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00113-015-0085-9"},{"key":"e_1_2_8_3_1","first-page":"527","article-title":"Physiopathology of osteoporosis: from risk factors analysis to treatment.","volume":"29","author":"Del Puente A.","year":"2015","journal-title":"J. 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