{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T23:28:06Z","timestamp":1770334086723,"version":"3.49.0"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T00:00:00Z","timestamp":1503532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011912","name":"Washington University School of Medicine in St. Louis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011912","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007496","name":"Biomedical Laboratory Research and Development, VA Office of Research and Development","doi-asserted-by":"publisher","award":["I01BX007080"],"award-info":[{"award-number":["I01BX007080"]}],"id":[{"id":"10.13039\/100007496","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>\n                    Male exposure to cigarette smoke is associated with seminal defects and with congenital anomalies and childhood cancers in offspring. In mice, paternal exposure to cigarette smoke condensate (CSC) causes molecular defects in germ cells and phenotypic effects in their offspring. Here we used an\n                    <jats:italic>ex vivo<\/jats:italic>\n                    testicular explant model and\n                    <jats:italic>in vivo<\/jats:italic>\n                    exposure to determine the concentration at which CSC impairs spermatogenesis and offspring development. We explanted testis tissue at postnatal day (P)5.5 and cultured it until P11.5. Assessment of growth parameters by analyzing expression of cell\u2010specific markers revealed that the explant system maintained structural and functional integrity. We exposed the P5.5 to \u201011.5 explants to various concentrations (40\u2010160 \u03bc\n                    <jats:bold>g\/ml) of CSC and confirmed that nicotine in the CSC was metabolized to cotinine. We assessed various growth and differentiation parameters, as well as testosterone production, and observed that many spermatogenesis features were impaired at 160<\/jats:bold>\n                    \u03bc\n                    <jats:bold>g\/ml CSC. The same parameters were impaired by a similar CSC concentration<\/jats:bold>\n                    in\n                    <jats:italic>vivo<\/jats:italic>\n                    .\n                    <jats:bold>Finally, females mated to males that were exposed to 160<\/jats:bold>\n                    \u03bc\n                    <jats:bold>\n                      g\/ml CSC neonatally had increased rates of pup resorption. We conclude that male exposure to CSC impairs offspring development and that the concentration at which CSC impairs spermatogenesis is similar\n                      <jats:italic>in vivo<\/jats:italic>\n                      and\n                      <jats:italic>ex vivo<\/jats:italic>\n                    <\/jats:bold>\n                    .\n                    <jats:bold>Given that the concentrations of CSC we used contained similar doses of nicotine as human smokers are exposed to, we argue that our model mimics human male reproductive effects of smoking.\u2014Esakky, P., Hansen, D. A., Drury, A. M., Felder, P., Cusumano, A., Moley, K. H. Testicular cells exhibit similar molecular responses to cigarette smoke condensate<\/jats:bold>\n                    <jats:italic>ex vivo<\/jats:italic>\n                    <jats:bold>and<\/jats:bold>\n                    in\n                    <jats:italic>vivo<\/jats:italic>\n                    . FASEB J. 32, 63\u201072 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700405r","type":"journal-article","created":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T20:50:16Z","timestamp":1503607816000},"page":"63-72","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Testicular cells exhibit similar molecular responses to cigarette smoke condensate ex\n                    <i>vivo<\/i>\n                    and\n                    <i>in vivo<\/i>"],"prefix":"10.1096","volume":"32","author":[{"given":"Prabagaran","family":"Esakky","sequence":"first","affiliation":[{"name":"Reasearch and Development Department of Veterans Affairs Medical Center St. Louis Missouri USA"},{"name":"Department of Obstetrics and Gynecology Washington University School of Medicine St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah A.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Reasearch and Development Department of Veterans Affairs Medical Center St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea M.","family":"Drury","sequence":"additional","affiliation":[{"name":"Reasearch and Development Department of Veterans Affairs Medical Center St. Louis Missouri USA"},{"name":"Department of Obstetrics and Gynecology Washington University School of Medicine St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Felder","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Washington University School of Medicine St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Cusumano","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Washington University School of Medicine St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelle H.","family":"Moley","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Washington University School of Medicine St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,24]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"The Health Conseqquences of Smoking\u201450 Years of Progress: A Report of the Surgeon General","author":"U.S. Department of Health and Human Services","year":"2014"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1016\/j.fertnstert.2012.07.1146","article-title":"Smoking and infertility: a committee opinion.","volume":"98","author":"The Practice Committee of the American Society for Reproductive Medicine","year":"2012","journal-title":"Fertil. Steril."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/21.2.273"},{"key":"e_1_2_8_5_1","volume-title":"TheChemicalComponentsof Tobacco and Tobacco Smoke","author":"Rodgman A.","year":"2009"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2004.05162.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2010.05.002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del364"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)61388-8"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2012.10.005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2016.08.017"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02144424"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-0279"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230600713475"},{"key":"e_1_2_8_15_1","volume-title":"Histological and Histopathological Evaluation of the Testis","author":"Russell L. D.","year":"1990"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1006\/scdb.1998.0203","article-title":"The central role of Sertoli cells in spermatogenesis.","volume":"9","author":"Griswold M. D.","year":"1998","journal-title":"Semin. CellDev. Biol."},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2001.tb03430.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-006-0167-7"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.138"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2006.09.022"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.11870"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0130171"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2012.03.016"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/tobaccocontrol-2012-050860"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-4096.2001.02384.x"},{"key":"e_1_2_8_26_1","first-page":"30","article-title":"On the toxicological assessment ofthe insecticide nicotine sulfate.","volume":"34","author":"Lazutka F. A.","year":"1969","journal-title":"Gig. Sanit."},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-013-1127-0"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.904235"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719112452939"},{"key":"e_1_2_8_30_1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1093\/aje\/kwm128","article-title":"Risk of spontaneous abortion in women with childhood exposure to parental cigarette smoke.","volume":"166","author":"Meeker J. D.","year":"2007","journal-title":"Am. J. Epidemiol."},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.108845"},{"key":"e_1_2_8_32_1","first-page":"1","article-title":"Effect of the anti\u2010oxidant tempol on fetal growth in a mouse model of fetal growth restriction.","volume":"87","author":"Stanley J. L.","year":"2012","journal-title":"Biol. Rieprod."},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03408.x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1177\/25.7.893996"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb02114.x"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0930233"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00245.x"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510813103"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.064337"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/4.3.227"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh565"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.12.019"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1994.1152"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-9-38"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.5.1303"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.5.5110"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(20000201)258:2<210::AID-AR10>3.0.CO;2-X"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-199310000-00002"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)66968-4"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024672522802"},{"key":"e_1_2_8_51_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/j.1439-0272.2004.00605.x","article-title":"Effects of paternal cigarette smoking on testicular function, sperm fertilizing capacity, embryonic development, and blastocyst capacity for implantation in rats.","volume":"36","author":"Kapawa A.","year":"2004","journal-title":"Andrologia"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.15.1.8679227"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00239.2007"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(90)90032-M"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq149"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.9490"},{"key":"e_1_2_8_57_1","first-page":"323","article-title":"Smoking and male reproduction: a review. Int. J.","volume":"19","author":"Vine M. F.","year":"1996","journal-title":"Androl."},{"key":"e_1_2_8_58_1","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1001\/jama.1995.03520340051035","article-title":"The effect of passive smoking and tobacco exposure through breast milk on sudden infant death syndrome.","volume":"273","author":"Klonoff\u2010Cohen H. S.","year":"1995","journal-title":"JAMA"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei080"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq103"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:30:2:213"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201700405r","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201700405r","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201700405r","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T20:04:24Z","timestamp":1761681864000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201700405r"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,24]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1096\/fj.201700405r"],"URL":"https:\/\/doi.org\/10.1096\/fj.201700405r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,24]]},"assertion":[{"value":"2017-08-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-05-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}