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Despite progress in understanding osteocyte biology and function, much remains to be elucidated. Recently developed osteocytic cell lines\u2014together with new genome editing tools\u2014has allowed a closer look at the biology and molecular makeup of these cells. By using single\u2010cell cloning, we identified genes that are associated with high Sost\/sclerostin expression and analyzed their regulation and function. Unbiased transcriptome analysis of high\u2010\n                    <jats:italic>vs.<\/jats:italic>\n                    low\u2010Sost\/ sclerostin\u2010expressing cells identified known and novel genes. Dmp1 (dentin matrix protein 1), Dkk1 (Dickkopf WNT signaling pathway inhibitor 1), and Phex were among the most up\u2010regulated known genes, whereas Srpx2, Cd200, and carbonic anhydrase III (CAIII) were identified as novel markers of differentiated osteocytes. Aspn, Enpp2, Robo2, Nov, and Serpina3g were among the transcripts that were most significantly suppressed in high\u2010Sost cells. Considering that CAII was recently identified as being regulated by Sost\/sclerostin and capable of controlling mineral homeostasis, we focused our attention on CAIII. Here, we report that CAIII is highly expressed in osteocytes, is regulated by parathyroid hormone both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    , and protects osteocytes from oxidative stress.\u2014Shi, C., Uda, Y., Dedic, C., Azab, E., Sun,N.,Hussein,A.I.,Petty,C.A.,Fulzele,K.,Mitterberger\u2010Vogt,M.C.,Zwerschke,W.,Pereira,R.,Wang,K.,Divieti Pajevic, P. Carbonic anhydrase III protects osteocytes from oxidative stress. FASEB J. 32,440\u2010452 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700485rr","type":"journal-article","created":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T20:50:23Z","timestamp":1505854223000},"page":"440-452","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Carbonic anhydrase III protects osteocytes from oxidative stress"],"prefix":"10.1096","volume":"32","author":[{"given":"Chao","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an China"},{"name":"Molecular and Cell Biology Henry M. Goldman School of Dental Medicine Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhei","family":"Uda","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology Henry M. Goldman School of Dental Medicine Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Dedic","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology Henry M. Goldman School of Dental Medicine Boston Massachusetts USA"},{"name":"Merks Laboratories Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehab","family":"Azab","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology Henry M. Goldman School of Dental Medicine Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningyuan","family":"Sun","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology Henry M. Goldman School of Dental Medicine Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira I.","family":"Hussein","sequence":"additional","affiliation":[{"name":"Department of Orthopedics School of Medicine, Boston University Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher A.","family":"Petty","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology Henry M. Goldman School of Dental Medicine Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keertik","family":"Fulzele","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology Henry M. Goldman School of Dental Medicine Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C.","family":"Mitterberger\u2010Vogt","sequence":"additional","affiliation":[{"name":"Cell Metabolism and Differentiation Research Group University of Innsbruck Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Zwerschke","sequence":"additional","affiliation":[{"name":"Cell Metabolism and Differentiation Research Group University of Innsbruck Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Pereira","sequence":"additional","affiliation":[{"name":"David Geffen School of Medicine University of California, Los Angeles Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunzheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola Divieti","family":"Pajevic","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology Henry M. 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