{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:52:36Z","timestamp":1790664756577,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,3]],"date-time":"2018-01-03T00:00:00Z","timestamp":1514937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008731","name":"Nakatomi Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:p>\n                    Apobec2 is a member of the activation\u2010induced deaminase\/apolipoprotein B mRNA editing enzyme catalytic polypeptide cytidine deaminase family expressed in differentiated skeletal and cardiac muscle. We previously reported that Apobec2 deficiency in mice leads to a shift in muscle fiber type, myopathy, and diminished muscle mass. However, the mechanisms of myopathy caused by Apobec2 deficiency and its physiologic functions are unclear. Here we show that, although Apobec2 localizes to the sarcomeric Z\u2010lines in mouse tissue and cultured myotubes, the sarcomeric structure is not affected in Apobec2\u2010deficient muscle. In contrast, electron microscopy reveals enlarged mitochondria and mitochondria engulfed by autophagic vacuoles, suggesting that Apobec2 deficiency causes mitochondrial defects leading to increased mitophagy in skeletal muscle. Indeed, Apobec2 deficiency results in increased reactive oxygen species generation and depolarized mitochondria, leading to mitophagy as a defensive response. Furthermore, the exercise capacity of\n                    <jats:italic>Apobec2<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice is impaired, implying Apobec2 deficiency results in ongoing muscle dysfunction. The presence of rimmed vacuoles in myofibers from 10\u2010mo\u2010old mice suggests that the chronic muscle damage impairs normal autophagy. We conclude that Apobec2 deficiency causes mitochondrial defects that increase muscle mitophagy, leading to myopathy and atrophy. Our findings demonstrate that Apobec2 is required for mitochondrial homeostasis to maintain normal skeletal muscle function.\u2014Sato, Y., Ohtsubo, H., Nihei, N., Kaneko, T., Sato, Y., Adachi, S.\u2010I., Kondo, S., Nakamura, M., Mizunoya, W., Iida, H., Tatsumi, R., Rada, C., Yoshizawa, F. Apobec2 deficiency causes mitochondrial defects and mitophagy in skeletal muscle. FASEB J. 32,1428\u20101439 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700493r","type":"journal-article","created":{"date-parts":[[2017,11,10]],"date-time":"2017-11-10T12:10:14Z","timestamp":1510315814000},"page":"1428-1439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Apobec2 deficiency causes mitochondrial defects and mitophagy in skeletal muscle"],"prefix":"10.1096","volume":"32","author":[{"given":"Yusuke","family":"Sato","sequence":"first","affiliation":[{"name":"Department of Agrobiology and Bioresources Utsunomiya University Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Ohtsubo","sequence":"additional","affiliation":[{"name":"Department of Animal and Marine Bioresource Sciences Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiro","family":"Nihei","sequence":"additional","affiliation":[{"name":"Department of Agrobiology and Bioresources Utsunomiya University Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takane","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Animal and Marine Bioresource Sciences Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoriko","family":"Sato","sequence":"additional","affiliation":[{"name":"United Graduate School of Agricultural Science Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin\u2010Ichi","family":"Adachi","sequence":"additional","affiliation":[{"name":"Department of Agrobiology and Bioresources Utsunomiya University Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Agrobiology and Bioresources Utsunomiya University Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mako","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Animal and Marine Bioresource Sciences Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Mizunoya","sequence":"additional","affiliation":[{"name":"Department of Animal and Marine Bioresource Sciences Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Iida","sequence":"additional","affiliation":[{"name":"Department of Animal and Marine Bioresource Sciences Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuichi","family":"Tatsumi","sequence":"additional","affiliation":[{"name":"Department of Animal and Marine Bioresource Sciences Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Rada","sequence":"additional","affiliation":[{"name":"Medical Research Council, Laboratory of Molecular Biology Cambridge United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiaki","family":"Yoshizawa","sequence":"additional","affiliation":[{"name":"Department of Agrobiology and Bioresources Utsunomiya University Tochigi Japan"},{"name":"United Graduate School of Agricultural Science Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8511591"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)36836-X"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00078-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.26.18470"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00939"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0925"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-6-229"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2014.01.003"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.16.7270-7277.2005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297911010135"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.90085"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.052977"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200912125"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.09.016"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.603043"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5603-11.2012"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1312009110"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.11.042"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.03.022"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26114"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00735.2009"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06639"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.12.018"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2009.10.008"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.10.005"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddr305"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.01.056"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114041"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2013.09.004"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2008.04.010"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"C1904","DOI":"10.1152\/ajpcell.2001.281.6.C1904","article-title":"ARCD\u20101, an apobec\u20101\u2010related cytidine deaminase, exerts a dominant negative effect on C to U RNA editing","volume":"281","author":"Anant S.","year":"2001","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.04.023"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2005.03.002"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2010.11.008"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3779"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3410233"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.21211"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.028"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2012"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000298"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201008084"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp326"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021115-105011"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2010.50"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2012-0119"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.733410"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11910-003-0040-y"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a003147"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300021s"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.10.4684"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-005-0033-1"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2220"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.60"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.11.001"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2010.28608A"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-061509-131248"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.01.016"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.22632"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2247"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003802"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2012.159"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201700493R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201700493R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201700493R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T23:15:16Z","timestamp":1761693316000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201700493R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,3]]},"references-count":61,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["10.1096\/fj.201700493R"],"URL":"https:\/\/doi.org\/10.1096\/fj.201700493r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,3]]},"assertion":[{"value":"2017-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}