{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:39:15Z","timestamp":1789713555847,"version":"4.0.1"},"reference-count":64,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,8,23]],"date-time":"2017-08-23T00:00:00Z","timestamp":1503446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005874","name":"Universit\u00e0 degli Studi G. d'Annunzio Chieti - Pescara","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005874","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recent research has widely investigated the anti\u2010inflammatory effects of mesenchymal stem cells and their secretory products, termed the secretome, in the treatment of multiple sclerosis (MS). The present study examined the capacity of the conditioned medium (CM) from human periodontal ligament stem cells (hPLSCs) under hypoxia (H\u2010hPDLSCs\u2010CM) to suppress experimental autoimmune encephalomyelitis (EAE), a murine model of MS. To induce EAE, female C57BL\/6 mice were immunized with myelin oligodendroglial glycoprotein peptide\n                    <jats:sub>35\u201355<\/jats:sub>\n                    . At the onset of symptoms, H\u2010hPDLSCs\u2010CM was infused\n                    <jats:italic>via<\/jats:italic>\n                    the tail vein of mice. Our results demonstrate the efficacy of H\u2010hPDLSCs\u2010CM treatment in diminishing clinical and histologic disease score. A key finding from this study is the marked expression of anti\u2010inflammatory cytokine IL\u201037, paralleled by the suppression of proinflammatory cytokines in mice with EAE that were treated with H\u2010hPDLSCs\u2010CM. In addition, a consequent modulation of oxidative stress, autophagic, and apoptotic markers was observed in mice with EAE after hPDLSCs\u2010CM administration. In addition, to provide additional evidence of the molecular mechanisms that underlie H\u2010hPDLSCs\u2010CM, we investigated its therapeutic action in scratch injury\u2013exposed NSC\u201034 neurons, an\n                    <jats:italic>in vitro<\/jats:italic>\n                    model of injury. This model reproduces severe inflammation and oxidative stress conditions as observed after EAE damage.\n                    <jats:italic>In vitro<\/jats:italic>\n                    results corroborate the ability of hPDLSCs\u2010CM to modulate inflammatory, oxidative stress, and apoptotic pathways. Taken together, our findings suggest H\u2010hPDLSCs\u2010CM as a new pharmacologic opportunity for the management of MS.\u2014Giacoppo, S., Thangavelu, S. R., Diomede, F., Bramanti, P., Conti, P., Trubiani, O., Mazzon, E. Anti\u2010inflammatory effects of hypoxia\u2010preconditioned human periodontal ligament cell secretome in an experimental model of multiple sclerosis: a key role of IL\u201037. FASEB J. 31, 5592\u20135608 (2017).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700524r","type":"journal-article","created":{"date-parts":[[2017,8,23]],"date-time":"2017-08-23T20:10:56Z","timestamp":1503519056000},"page":"5592-5608","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Anti\u2010inflammatory effects of hypoxia\u2010preconditioned human periodontal ligament cell secretome in an experimental model of multiple sclerosis: a key role of IL\u201037"],"prefix":"10.1096","volume":"31","author":[{"given":"Sabrina","family":"Giacoppo","sequence":"first","affiliation":[{"name":"Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Centro Neurolesi Bonino\u2010Pulejo, Contrada Casazza Messina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soundara Rajan","family":"Thangavelu","sequence":"additional","affiliation":[{"name":"Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Centro Neurolesi Bonino\u2010Pulejo, Contrada Casazza Messina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Diomede","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine Laboratory Department of Medical Oral, and Biotechnological Sciences University G. d'Annunzio Chieti Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Placido","family":"Bramanti","sequence":"additional","affiliation":[{"name":"Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Centro Neurolesi Bonino\u2010Pulejo, Contrada Casazza Messina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pio","family":"Conti","sequence":"additional","affiliation":[{"name":"Immunology Division Postgraduate Medical School University of Chieti\u2010Pescara Chieti Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriana","family":"Trubiani","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine Laboratory Department of Medical Oral, and Biotechnological Sciences University G. d'Annunzio Chieti Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Mazzon","sequence":"additional","affiliation":[{"name":"Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Centro Neurolesi Bonino\u2010Pulejo, Contrada Casazza Messina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-005-5002-7"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra052130"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161212799040501"},{"key":"e_1_2_9_5_1","first-page":"24","article-title":"Mesenchymal stem cells in multiple sclerosis\u2014translation to clinical trials","volume":"8","author":"Dulamea A.","year":"2015","journal-title":"J. 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