{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T16:57:15Z","timestamp":1780765035071,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,1,17]],"date-time":"2018-01-17T00:00:00Z","timestamp":1516147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009560","name":"Uniklinikum Giessen und Marburg","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009560","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009091","name":"Justus Liebig Universit\u00e4t Gie\u00dfen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009091","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We have recently identified a cholinergic chemosensory cell in the urethral epithelium, urethral brush cell (UBC), that, upon stimulation with bitter or bacterial substances, initiates a reflex detrusor activation. Here, we elucidated cholinergic mechanisms that modulate UBC responsiveness. We analyzed muscarinic acetylcholine receptor (M1\u20135 mAChR) expression by using RT\u2010PCR in UBCs, recorded [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    responses to a bitter stimulus in isolated UBCs of wild\u2010type and mAChR\u2010deficient mice, and performed cystometry in all involved strains. The bitter response of UBCs was enhanced by global cholinergic and selective M2 inhibition, diminished by positive allosteric modulation of M5, and unaffected by M1, M3, and M4 mAChR inhibitors. This effect was not observed in M2 and M5 mAChR\u2010deficient mice. In cystometry, M5 mAChR\u2010deficient mice demonstrated signs of detrusor overactivity. In conclusion, M2 and M5 mAChRs attenuate the bitter response of UBC\n                    <jats:italic>via<\/jats:italic>\n                    a cholinergic negative autocrine feedback mechanism. Cystometry suggests that dysfunction, particularly of the M5 receptor, may lead to such symptoms as bladder overactivity.\u2014Deckmann, K., Rafiq, A., Erdmann, C., Illig, C., Durschnabel, M., Wess, J., Weidner, W., Bschleipfer, T., Kummer, W. Muscarinic receptors 2 and 5 regulate bitter response of urethral brush cells\n                    <jats:italic>via<\/jats:italic>\n                    negative feedback. FASEB J. 32, 2903\u20132910 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700582r","type":"journal-article","created":{"date-parts":[[2018,1,25]],"date-time":"2018-01-25T14:33:21Z","timestamp":1516890801000},"page":"2903-2910","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Muscarinic receptors 2 and 5 regulate bitter response of urethral brush cells\n                    <i>via<\/i>\n                    negative feedback"],"prefix":"10.1096","volume":"32","author":[{"given":"Klaus","family":"Deckmann","sequence":"first","affiliation":[{"name":"Institute for Anatomy and Cell Biology Pediatric Urology, and Andrology Justus-Liebig-University Giessen  Giessen 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