{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T06:08:33Z","timestamp":1767161313437,"version":"build-2238731810"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T00:00:00Z","timestamp":1515024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31402184"],"award-info":[{"award-number":["31402184"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p>\n                    Translationally controlled tumor protein (TCTP) is a highly conserved, multifunctional protein that has been implicated in a range of cell physiologic processes, especially cell growth and development. A TCTP\u2010like gene has been identified in the\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    genome [\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    TCTP (\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP)], although its function remains unknown. The sequence analysis of\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP indicated that it is a highly conserved protein in eukaryotes. We found that the expression level of\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP in the virulent RH strain was significantly higher than that in the avirulent PLK strain. Indirect immunofluorescence showed that\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP was expressed in the parasite cytoplasm. The localization of\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP was unchanged during the replication of the parasite. We expressed a functional recombinant\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP (r\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP) protein in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and found that the recombinant protein could form a multimer. We then evaluated the function of\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP using the clustered regularly interspaced short palindromic repeats (CRISPR)\/ CRISPR\u2010associated protein 9 knockout (KO) system. Phenotypic analysis of the KO strain (\u0394\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP) revealed that\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP is required for the robust growth of the parasites.\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP deficiency also led to early egress of the parasites and subsequent impairment in their invasion and attachment abilities. We subsequently found that the multimer form of\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP might not be necessary for the growth and replication of the parasite. Then the expression profiling of genes in the \u0394\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP and complement strains were analyzed. The results revealed that 988 genes were regulated in \u0394\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP compared with the complement strain. Overall, although not essential,\n                    <jats:italic>Tg<\/jats:italic>\n                    TCTP is required for the fast growth of\n                    <jats:italic>Tg<\/jats:italic>\n                    and maintenance of its intracellular development.\u2014Zheng J., Chen, Y., Li, Z., Cao, S., Zhang Z., Jia, H. Translationally controlled tumor protein is required for the fast growth of\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    and maintenance of its intracellular development.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    32, 906\u2013919 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201700994r","type":"journal-article","created":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T18:00:11Z","timestamp":1508349611000},"page":"906-919","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Translationally controlled tumor protein is required for the fast growth of\n                    <i>Toxoplasma gondii<\/i>\n                    and maintenance of its intracellular 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Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoxia","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honglin","family":"Jia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 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