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Brain pericytes play a vital role in maintaining BBB integrity by interacting with other components of the BBB. In this study, we found that sphingosine\u20101\u2010phosphate receptor (S1PR)2 expressed in pericytes was significantly up\u2010regulated after ischemia in\n                    <jats:italic>vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro.<\/jats:italic>\n                    By using a S1PR2 antagonist (JTE\u2010013), we showed that S1PR2 plays a critical role in the induction of BBB permeability of transient middle cerebral artery occlusion (tMCAO) rats and the in\n                    <jats:italic>vitro<\/jats:italic>\n                    BBB model. Furthermore, we discovered that S1PR2 may decrease N\u2010cadherin expression and increase pericyte migration\n                    <jats:italic>via<\/jats:italic>\n                    NF\u2010\u03baB p65 signal and found that S1PR2 could be regulated by miR\u2010149\u20135p negatively, which was decreased in the ischemic boundary zone and cultured pericytes after ischemia. Overexpression of miR\u2010149\u20135p in cultured pericytes substantially increased N\u2010cadherin expression and decreased pericyte migration, which decreased BBB leakage in the\n                    <jats:italic>in vitro<\/jats:italic>\n                    model. Up\u2010regulating miR\u2010149\u20135p by intracerebroventricular injection of agomir\u2010149\u20135p attenuated BBB permeability and improved the outcomes of tMCAO rats significantly. Thus, our data suggest that miR\u2010149\u20135p may serve as a potential target for treatment of BBB disruption after ischemic stroke.\u2014Wan, Y., Jin, H.\u2010J., Zhu, Y.\u2010Y., Fang Z., Mao, L., He, Q., Xia, Y.\u2010P., Li, M., Li, Y., Chen, X., Hu, B. MicroRNA\u2010149\u20135p regulates blood\u2010brain barrier permeability after transient middle cerebral artery occlusion in rats by targeting S1PR2 of pericytes. FASEB J. 32, 3133\u20133148 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201701121r","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T17:38:42Z","timestamp":1516297122000},"page":"3133-3148","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["MicroRNA\u2010149\u20135p regulates blood\u2013brain barrier permeability after transient middle cerebral artery occlusion in rats by targeting S1PR2 of pericytes"],"prefix":"10.1096","volume":"32","author":[{"given":"Yan","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Neurology, Union Hospital Tongji Medical College  Wuhan 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