{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:39:00Z","timestamp":1788172740431,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T00:00:00Z","timestamp":1523923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31528006"],"award-info":[{"award-number":["31528006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    During its life cycle, Zika virus (ZIKV), an arthropod\u2010borne flavivirus that is associated with Guillain\u2010Barr\u00e9 syndrome and causes microencephaly in fetuses and newborn children, encodes a critical and indispensable helicase domain that has 5'\u2010triphosphatase activity and performs ATP hydrolysis to generate energy and thus, sustains unwinding of double\u2010stranded RNA during ZIKV genome replication. Of these processes, ATP hydrolysis represents the most basic event; however, its dynamic mechanisms remain largely unknown, impeding the further understanding of the function of ZIKV helicase and the ongoing anti\u2010ZIKV drug design. In this work, we determined the crystal structure of ZIKV helicase in complex with ADP\u2010AlF\n                    <jats:sub>3<\/jats:sub>\n                    \u2010Mn\n                    <jats:sup>2+<\/jats:sup>\n                    and ADP\u2010Mn\n                    <jats:sup>2+<\/jats:sup>\n                    separately. The structural analysis indicates that these structures represent the intermediate state and posthydrolysis state, respectively, of the ATP hydrolysis process of ZIKV helicase. These findings, together with our earlier work, which identified the prehydrolysis state of ZIKV helicase, lead to a proposal of the ATP hydrolysis cycle for ZIKV helicase. On this basis, we used site\u2010 directed mutagenesis combined with an enzymatic study to identify successfully residues that are critical for the ATPase activity of ZIKV helicase; this will provide new ideas to understand the function for the key enzyme of ZIKV.\u2014Yang, X., Chen, C., Tian, H., Chi, H., Mu, Z., Zhang, T., Yang, K., Zhao, Q., Liu, X., Wang, Z., Ji, X., Yang, H. Mechanism of ATP hydrolysis by the Zika virus helicase.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    32, 5250\u20135257 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201701140r","type":"journal-article","created":{"date-parts":[[2018,4,25]],"date-time":"2018-04-25T16:01:06Z","timestamp":1524672066000},"page":"5250-5257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Mechanism of ATP hydrolysis by the Zika virus helicase"],"prefix":"10.1096","volume":"32","author":[{"given":"Xiaoyun","family":"Yang","sequence":"first","affiliation":[{"name":"School of Life Sciences Tianjin University  Tianjin China"},{"name":"Tianjin International Joint Academy of Biotechnology and Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongliang","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Life Sciences Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Chi","sequence":"additional","affiliation":[{"name":"School of Life Sciences Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyu","family":"Mu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kailin","family":"Yang","sequence":"additional","affiliation":[{"name":"Cleveland Clinic Lerner College of Medicine Case Western Reserve University  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Molecular Biophysics and Biochemistry Yale University  New Haven Connecticut USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"Liu","sequence":"additional","affiliation":[{"name":"The State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zefang","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Ji","sequence":"additional","affiliation":[{"name":"The State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haitao","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Tianjin University  Tianjin China"},{"name":"Tianjin International Joint Academy of Biotechnology and Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.7326\/M16-0150"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(52)90042-4"},{"key":"e_1_2_7_4_1","first-page":"747","volume-title":"Fields Virology","author":"Pierson T. 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