{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:50:42Z","timestamp":1789167042912,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T00:00:00Z","timestamp":1516579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM62116"],"award-info":[{"award-number":["GM62116"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009250","name":"National Institute for Social Care and Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009250","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["WT107964MA"],"award-info":[{"award-number":["WT107964MA"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006396","name":"Alexion Pharmaceuticals","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006396","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Patients with cystic fibrosis (CF) experience chronic or recurrent bacterial and fungal lung infections. Many patients with CF cannot effectively clear\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    from their lungs. This may result in IgE sensitization and the development of allergic bronchopulmonary aspergillosis, or invasive infections, such as\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    bronchitis. Lung disease in patients with CF is associated with neutrophil\u2010dominated inflammation and elevated levels of the serine protease, neutrophil elastase (NE). Various C\u2010type lectin\u2010like receptors (CLRs), including Dectin\u20101 and Dectin\u20102, are involved in the immune response to\n                    <jats:italic>Aspergillus.<\/jats:italic>\n                    Here, we show that purified NE cleaves Dectin\u20101 in an isoform\u2010specific manner. Bronchoalveolar lavage fluid from patients with CF, which contains high NE activity, induces Dectin\u20101 cleavage. Similarly, filtrate from a protease\u2010producing strain of\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    induces isoform\u2010specific cleavage of Dectin\u20101. Dectin\u20101 knockout (KO) cells and NE\u2010treated cells demonstrated reduced phagocytosis of zymosan, a fungal cell wall preparation. In addition, NE cleaves 2 other CLRs, Dectin\u20102 and Mincle, and fungal\u2010induced cytokine production was reduced in Dectin\u20101 KO cells, Dectin\u20102 KO cells, and NE\u2010treated cells. Thus, Dectin\u20101 and Dectin\u20102 cleavage by NE and\/or\n                    <jats:italic>A.fumigatus<\/jats:italic>\n                    \u2013derived proteases results in an aberrant antifungal immune response that likely contributes to disease pathology in patients with CF.\u2014Griffiths J. S., Thompson, A., Stott, M., Benny, A., Lewis, N. A., Taylor, P. R., Forton, J., Herrick, S., Orr, S. J., McGreal E. P. Differential susceptibility of Dectin\u20101 isoforms to functional inactivation by neutrophil and fungal proteases. FASEB J. 32, 3385\u20133397 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201701145r","type":"journal-article","created":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T22:15:53Z","timestamp":1516659353000},"page":"3385-3397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Differential susceptibility of Dectin\u20101 isoforms to functional inactivation by neutrophil and fungal proteases"],"prefix":"10.1096","volume":"32","author":[{"given":"James S.","family":"Griffiths","sequence":"first","affiliation":[{"name":"Division of Infection and Immunity Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiysha","family":"Thompson","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Stott","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Benny","sequence":"additional","affiliation":[{"name":"School of Medicine, Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie A.","family":"Lewis","sequence":"additional","affiliation":[{"name":"School of Medicine, Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip R.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Forton","sequence":"additional","affiliation":[{"name":"Children's Hospital for Wales  Cardiff United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Herrick","sequence":"additional","affiliation":[{"name":"Centre for Medical Education Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selinda J.","family":"Orr","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eamon P.","family":"McGreal","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Faculty of Biology Medicine and Health Manchester Academic Health Science Centre University of Manchester  Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2016.04.022"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.150.6.7952609"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.04.007"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-014-0462-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00301.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0802629"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0804250"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.6.3717"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0010042"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-04-279307"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300748"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwj077"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004643"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2797"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3855\/jidc.7570","article-title":"The role of Mincle in innate immune to fungal keratitis","volume":"11","author":"Zhao G.","year":"2017","journal-title":"J. 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