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At this time, there are no identified biomarkers to demarcate asbestos exposure before the presentation of disease and symptoms, and there is only limited understanding of the underlying biology that governs asbestos\u2010induced disease. In our study, we used exosomes, 30\u2010140 nm extracellular vesicles, to gain insight into these knowledge gaps. As inhaled asbestos is first encountered by lung epithelial cells and macrophages, we hypothesize that asbestos\u2010exposed cells secrete exosomes with signature proteomic cargo that can alter the gene expression of mesothelial cells, contributing to disease outcomes like mesothelioma. In the present study using lung epithelial cells (BEAS2B) and macrophages (THP\u20101), we first show that asbestos exposure causes changes in abundance of some proteins in the exosomes secreted from these cells. Furthermore, exposure of human mesothelial cells (HPM3) to these exosomes resulted in gene expression changes related to epithelial\u2010to\u2010mesenchymal transition and other cancer\u2010related genes. This is the first report to indicate that asbestos\u2010exposed cells secrete exosomes with differentially abundant proteins and that those exosomes have a gene\u2010altering effect on mesothelial cells.\u2014Munson, P., Lam, Y.\u2010W., Dragon, J. MacPherson, M., Shukla, A. 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