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Its mechanism is still unknown, but learning how to improve the BTB permeability is critical for drug intervention. Recently, microRNAs (miRNAs) have appeared as regulation factors of numerous biologic processes and therapeutic targets of diverse diseases. In this study, we have identified that miR\u2010132\u20103p is an essential miRNA by increasing the transcellular transport through the BTB. We found that miR\u2010132\u20103p expression was significantly up\u2010regulated in glioma endothelial cells (GECs). Furthermore we showed that miR132\u20103p\n                    <jats:sup>+<\/jats:sup>\n                    greatly induced the endocytosis of cholera toxin subunit B and FITC\u2013bovine serum albumin and up\u2010regulated the expression of p\u2010PKB, p\u2010Src and Tyr14 phosphorylation of caveolin\u20101 (p\u2010Cav\u20101), while phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression was markedly down\u2010regulated in GECs. Our results identify PTEN as a direct and functional downstream target of miR\u2010132\u20103p, which is involved in the regulation of p\u2010PKB, p\u2010Src, and p\u2010Cav\u20101. The inhibitors for PI3K and Src significantly reversed the increase of p\u2010Cav\u20101 induced by miR\u2010132\u20103p. Moreover, overexpression of PTEN greatly reduced the endocytosis of cholera toxin subunit B and the up\u2010regulation of p\u2010Cav\u20101 induced by agomiR132\u20103p, suggesting that miR132\u20103p\n                    <jats:sup>+<\/jats:sup>\n                    increases the endothelial permeability by inhibition of PTEN expression. In addition, miR132\u20103p\n                    <jats:sup>+<\/jats:sup>\n                    significantly increased the delivery of doxorubicin across the BTB\n                    <jats:italic>in vitro<\/jats:italic>\n                    and contributed to the accumulation of doxorubicin within the brain tumor tissue. Our results show that miR\u2010132\u20103p contributes to the increased permeability of BTB by targeting PTEN\/PI3K\/PKB\/Src\/Cav\u20101, thereby revealing a novel drug target for the treatment of brain gliomas.\u2014Gu, Y., Cai, R., Zhang, C., Xue, Y., Pan, Y., Wang, J., Zhang, Z. miR\u2010132\u20103p boosts caveolae\u2010mediated transcellular transport in glioma endothelial cells by targeting PTEN\/PI3K\/PKB\/Src\/Cav\u20101 signaling pathway. FASEB J. 33, 441\u2013454 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201800095rr","type":"journal-article","created":{"date-parts":[[2018,7,19]],"date-time":"2018-07-19T14:01:29Z","timestamp":1532008889000},"page":"441-454","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["miR\u2010132\u20103p boosts caveolae\u2010mediated transcellular transport in glioma endothelial cells by targeting PTEN\/PI3K\/PKB\/Src\/Cav\u20101 signaling pathway"],"prefix":"10.1096","volume":"33","author":[{"given":"Yanting","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Physiology Life Science and Biopharmaceutical Institution Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiping","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Physiology Life Science and Biopharmaceutical Institution Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology Life Science and Biopharmaceutical Institution Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixue","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Neurobiology College Basic of Medicine China Medical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Physiology Life Science and Biopharmaceutical Institution Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology Life Science and Biopharmaceutical Institution Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology Life Science and Biopharmaceutical Institution Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311-017-0522-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2017.02.009"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-9785-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700786RR"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2014.12.029"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12708"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700139RRR"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-017-0856-5"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X15606462"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201600435RR"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2017.69"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.08.004"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-016-2173-2"},{"key":"e_1_2_8_15_1","first-page":"3211","article-title":"miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro","volume":"8","author":"Wang H.","year":"2015","journal-title":"OncoTargets Ther."},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-2541-5"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10557-014-6533-x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/01902148.2015.1004206"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-238527"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2186"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16669365"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32180"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2016.05.006"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2309"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","unstructured":"Zhao Y. 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