{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T01:47:26Z","timestamp":1776995246850,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:00:00Z","timestamp":1540339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["HI12C0050"],"award-info":[{"award-number":["HI12C0050"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["HI15C0001"],"award-info":[{"award-number":["HI15C0001"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["HC15C1129"],"award-info":[{"award-number":["HC15C1129"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2015R1A2A1A15053374"],"award-info":[{"award-number":["NRF-2015R1A2A1A15053374"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2017R1A2B2005849"],"award-info":[{"award-number":["NRF-2017R1A2B2005849"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002630","name":"Ewha Womans University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002630","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Phenotype transition of mesothelial cells, such as epithelial\u2010to\u2010mesenchymal transition (EMT), is one of the early mechanisms of peritoneal fibrosis, which is mediated by oxidative stress and inflammation. Nucleotide\u2010binding oligomerization domain\u2010like receptor family pyrin domain containing 3 (NLRP3) inflammasome is a multiprotein oligomer that promotes the maturation of IL\u2010l\u03b2 and IL\u201018. Paricalcitol is reported to exert an antiinflammatory effect; however, there are no studies as to whether paricalcitol modulates the activation of NLRP3 inflammasome. We investigated the role of NLRP3 inflammasome in peritoneal EMT with an exploration of the effect of paricalcitol on oxidative stress, NLRP3 inflammasome, and EMT of mesothelial cells. TGF\u2010\u03b2l\u2010induced EMT in human peritoneal mesothelial cells (HPMCs) was associated with an up\u2010regulation of NLRP3, apoptosis\u2010associated speck\u2010like protein containing a caspase recruitment domain (ASC), and \u03c1rocas\u03c1ase\u20101, with an increased production of IL\u2010l\u03b2 and IL\u201018, which was ameliorated by small interfering (si)NLRP3, siASC, caspase inhibitors, or neutralizing antibodies for IL\u2010l\u03b2 and IL\u201018. TGF\u2010\u03b2l enhanced reactive oxygen species generation with an increase in NADPH oxidase (NOX) activity and mitochondrial NOX4 production. Paricalcitol alleviated TGF\u2010\u03b2l\u2010induced EMT and the NLRP3 inflammasome, which was associated with a down\u2010regulation of NOX activity by interfering with p47phox and p22phox interaction and mitochondrial NOX4 production in HPMCs. Taken together, paricalcitol ameliorated EMT of HPMCs\n                    <jats:italic>via<\/jats:italic>\n                    modulating an NOX\u2010dependent increase in the activity of NLRP3 inflammasome. Paricalcitol could be a novel approach to protect the peritoneum from the development of EMT and peritoneal fibrosis.\u2014Ko, J., Kang, H.\u2010J., Kim, D.\u2010A., Ryu, E.\u2010S., Yu, M., Lee, H., Lee, H. K., Ryu, H.\u2010M., Park, S.\u2010H., Kim, Y.\u2010L., Kang, D.\u2010H. Paricalcitol attenuates TGF\u2010\u03b2l\u2013induced phenotype transition of human peritoneal mesothelial cells (HPMCs) via modulation of oxidative stress and NLRP3 inflammasome. FASEB J. 33, 3035\u20133050 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201800292rr","type":"journal-article","created":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T13:40:10Z","timestamp":1540388410000},"page":"3035-3050","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Paricalcitol attenuates TGF\u2010\u03b21\u2013induced phenotype transition of human peritoneal mesothelial cells (HPMCs)\n                    <i>via<\/i>\n                    modulation of oxidative stress and NLRP3 inflammasome"],"prefix":"10.1096","volume":"33","author":[{"given":"Jiyeon","family":"Ko","sequence":"first","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Ewha Womans University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun-Jung","family":"Kang","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Ewha Womans University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dal-Ah","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Ewha Womans University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun-Sun","family":"Ryu","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Ewha Womans University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mina","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seonam Hospital Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huisong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Surgery Ewha Medical Research Center Ewha Womans University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyeon Kook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Surgery Ewha Medical Research Center Ewha Womans University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye-Myung","family":"Ryu","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine School of Medicine Kyungpook National University Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun-Hee","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine School of Medicine Kyungpook National University Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Lim","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine School of Medicine Kyungpook National University Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duk-Hee","family":"Kang","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Ewha Womans University School of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V132470"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020809"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00861.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2009.436"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1177\/089686080302300604","article-title":"Basic mechanisms and clinical implications of peritoneal fibrosis","volume":"23","author":"Margetts P. 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