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An unresolved question is to what extent such age\u2010related alterations in circadian organization are driven by the central clock in the suprachiasmatic nucleus (SCN), modifying timing signals to contributing peripheral tissue oscillators, and are mediated by underlying changes in the local cellular oscillators themselves. Using a bioluminescence reporter approach, we sought to determine whether circadian clock function in human adipocytes from subcutaneous (SAT) and visceral (VAT) adipose tissues changes with age. SAT and VAT biopsies were obtained from obese individuals during gastric bypass surgeries [\n                    <jats:italic>n<\/jats:italic>\n                    = 16; body mass index: 44.8 \u00b1 11.4 kg\/m\n                    <jats:sup>2<\/jats:sup>\n                    ; age: 44 \u00b1 9 yr (range: 30\u201358)]. Cells were isolated and transduced with a lentiviral circadian reporter construct [brain and muscle ary1 hydrocarbon receptor nuclear translocator\u2010like:luciferase (\n                    <jats:italic>BMAL:LUC<\/jats:italic>\n                    )], and bioluminescence was recorded over a period of 3 d. Human\n                    <jats:italic>BMAL1:LUC<\/jats:italic>\n                    adipocytes displayed a robust luminescence rhythm with comparable within\u2010individual periods in mature and preadipocytes (\n                    <jats:italic>P<\/jats:italic>\n                    &gt; 0.05). With increasing age, the circadian period decreased in mature adipocytes (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.005) (\u03b2 = 4 min\/yr;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). Our\n                    <jats:italic>ex vivo<\/jats:italic>\n                    approach indicated that ageing changes the organization of endogenous circadian oscillators in human adipocytes, independent of SCN signaling.\u2014Kolbe, I., Carrasco\u2010Benso, M. P., L\u00f3pez\u2010M\u00ednguez, J., Luj\u00e1n, J., Scheer, F. A. J. L., Oster, H., Garaulet, M. Circadian period of luciferase expression shortens with age in human mature adipocytes from obese patients. FASEB J. 33, 175\u2013180 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201800441r","type":"journal-article","created":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T16:08:59Z","timestamp":1530547739000},"page":"175-180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Circadian period of luciferase expression shortens with age in human mature adipocytes from obese patients"],"prefix":"10.1096","volume":"33","author":[{"given":"Isa","family":"Kolbe","sequence":"first","affiliation":[{"name":"Institute of Neurobiology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Paz","family":"Carrasco-Benso","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Biology University of Murcia  Murcia Spain"},{"name":"Biomedical Research Institute-Arrixaca  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"L\u03ccpez-M\u00ednguez","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Biology University of Murcia  Murcia Spain"},{"name":"Biomedical Research Institute-Arrixaca  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Luj\u00e1n","sequence":"additional","affiliation":[{"name":"General Surgery Service University Hospital Virgen de la Arrixaca  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank A. 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