{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:54:38Z","timestamp":1787064878627,"version":"build-2736575974"},"reference-count":82,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,6,7]],"date-time":"2018-06-07T00:00:00Z","timestamp":1528329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Schistosomiasis is a human parasitic disease caused by flatworms of the genus\n                    <jats:italic>Schistosoma.<\/jats:italic>\n                    Adult female schistosomes produce numerous eggs that are responsible for the pathogenesis and transmission of the disease, and the maturation of female gonads depends on the permanent pairing of females and males. Signaling protein kinases have been proven to control female gonad differentiation after pairing; however, little is known about the roles of protein phosphatases in the developmental and reproductive biology of schistosomes. Here we explored 3 genes encoding catalytic subunits of serine\/threonine protein phosphatase 1 (PP1c) that were structurally and evolutionarily conserved in\n                    <jats:italic>Schistosoma japonicum. In situ<\/jats:italic>\n                    hybridization showed transcripts of 3\n                    <jats:italic>Sj\u2010pp1c<\/jats:italic>\n                    genes mainly localized in the reproductive organs and tissues. Triple knockdown of\n                    <jats:italic>Sj\u2010pp1c<\/jats:italic>\n                    genes by RNA interference caused stunted growth and decreased pairing stability of worm pairs, as well as a remarkable reduction in cell proliferation activity and defects in reproductive maturation and fecundity. Transcriptomic analysis post\u2013RNA interference suggested that\n                    <jats:italic>Sj\u2010pp1c<\/jats:italic>\n                    genes are involved in controlling worm development and maturation mainly by regulating cell proliferation, eggshell synthesis, nutritional metabolism, cytoskeleton organization, and neural process. Our study provides the first insight into the fundamental contribution of\n                    <jats:italic>Sj<\/jats:italic>\n                    \u2010PP1c to molecular mechanisms underlying the reproductive biology of schistosomes.\u2014Zhao, L., Lu, Z., He, X., Mughal, M. N., Fang, R., Zhou, Y., Zhao, J., Gasser, R. B., Grevelding, C. G., Ye, Q., Hu, M. Serine\/threonine protein phosphatase 1 (PP1) controls growth and reproduction in\n                    <jats:italic>Schistosoma japonicum<\/jats:italic>\n                    .\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    32, 6626\u20136642 (2018).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"htto:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201800725r","type":"journal-article","created":{"date-parts":[[2018,6,7]],"date-time":"2018-06-07T17:29:10Z","timestamp":1528392550000},"page":"6626-6642","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Serine\/threonine protein phosphatase 1 (PP1) controls growth and reproduction in\n                    <i>Schistosoma japonicum<\/i>"],"prefix":"10.1096","volume":"32","author":[{"given":"Lu","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine, Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biomedical Research Center Seltersberg (BFS) Justus Liebig University Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine, Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mudassar N.","family":"Mughal","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine, Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine, Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqin","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine, Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junlong","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine, Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine, Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph G.","family":"Grevelding","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biomedical Research Center Seltersberg (BFS) Justus Liebig University Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine, Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine, Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,7]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"53","article-title":"Schistosomiasis: number of people treated worldwide in 2014","volume":"91","author":"World Health Organization","year":"2016","journal-title":"Wkly. Epidemiol. Rec."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.12.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000504"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.01.012"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1799-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(01)01893-1"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182010000053"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006147"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2017.12.001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(96)10060-8"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.2.241"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00013.2003"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075766"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.135376"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874467208666150126154222"},{"key":"e_1_2_8_17_1","volume-title":"Cultivation in Vitro of Medical Parasite","author":"Chen P. H.","year":"1995"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2010.08.008"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.13.3097"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.263"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5769(97)00008-1"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2524-7"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11924"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp120"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.016"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-2-r14"},{"key":"e_1_2_8_31_1","volume-title":"Research Design and Statistical Analysis","author":"Myers J. L.","year":"2010"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.10.006"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2012.08509.x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004246"},{"key":"e_1_2_8_35_1","first-page":"561","article-title":"Studies on the cultivation of newly laid eggs of Schistosoma japonicum in vitro","volume":"20","author":"Zhong F.","year":"2004","journal-title":"Chin. J. Zoonoses"},{"key":"e_1_2_8_36_1","first-page":"21","volume-title":"Protein Phosphatases","author":"Dombradi V.","year":"2003"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2014.07.001"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpev.1998.0560"},{"key":"e_1_2_8_39_1","first-page":"123","article-title":"Conservation of male\u2010specific expression of novel phosphoprotein phosphatases in Drosophila","volume":"220","author":"Adam C.","year":"2010","journal-title":"Dev. GenesEvol."},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.110239"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14693"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.9100"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.106.069914"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.04.109"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.130138"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.01.020"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2009.06.005"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2009.08.003"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.3.1846"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-1-5"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8730"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1350-1358.2000"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20051597"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.05.026"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31150"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2014.00170"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.02.037"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609708104"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2011.03.001"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2016.03.004"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(96)02703-X"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040014"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.06.007"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.10.009"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003856"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2007.11.017"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001907"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00535-8"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00364-X"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762006000900032"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003254"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2013.07.008"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066396"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2014.09.006"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00405-4"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-2959-x"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1139\/Z09-126"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2012.11.008"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003878"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004684"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00847"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.2007.B0498"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000334"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201800725R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201800725R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201800725R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T19:26:12Z","timestamp":1761679572000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201800725R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,7]]},"references-count":82,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1096\/fj.201800725R"],"URL":"https:\/\/doi.org\/10.1096\/fj.201800725r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,7]]},"assertion":[{"value":"2018-04-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}