{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T16:05:01Z","timestamp":1769270701963,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,6]],"date-time":"2018-12-06T00:00:00Z","timestamp":1544054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370798"],"award-info":[{"award-number":["81370798"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670633"],"award-info":[{"award-number":["81670633"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770686"],"award-info":[{"award-number":["81770686"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003397","name":"Huazhong University of Science and Technology","doi-asserted-by":"publisher","award":["2017KFYXJJ101"],"award-info":[{"award-number":["2017KFYXJJ101"]}],"id":[{"id":"10.13039\/501100003397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Protein tyrosine (Tyr) nitration, the covalent addition of a nitro group (\n                    <jats:sup>\u2022<\/jats:sup>\n                    NO\n                    <jats:sub>2<\/jats:sub>\n                    ) to Tyr residues, is emerging as a candidate mechanism of endothelial dysfunction. Previous studies have shown that Tyr nitration is primarily induced by nitrosative stress, a process characterized by the production of reactive nitrogen species, especially peroxynitrite anion (ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    ), which is considered a secondary product of NO in the presence of superoxide radicals (O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    ). However, the impact of nitrosative stress\u2013induced Tyr nitration on endothelial dysfunction has not been thoroughly elucidated to date. We developed an endothelial dysfunction model, a process called \u201cendothelial\u2010to\u2010mesenchymal transition (EndMT),\u201d and evaluated the production of NO, O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    , and protein nitration during EndMT. The results showed that TGF\u2010\u03b21 stimulation induced EndMT and elevated endothelial NO and O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    production as well as nitration of the catalytic subunit of protein phosphatase (PP)2A. Mass spectrometry analysis showed that Tyr265 was the nitration site in the catalytic subunit of protein phosphatase (PP)2A, and this Tyr nitration increased PP2A activity and disrupted endothelial integrity. To devise an endothelial\u2010targeted anti\u2010PP2Ac nitration strategy, a mimic peptide, tyrosine 265 wild type (Y265WT), conjugated with the cell\u2010penetrating peptide HIV\u20101 TAT protein (TAT) was synthesized. PP2Ac nitration and PP2A activity were significantly inhibited by pretreatment with TAT\u2010265WT, and the integrity of endothelial cells was maintained. Furthermore, injection of TAT\u2010265WT attenuated renal nitration formation and caused anticapillary rarefaction in a unilateral urethral obstructive nephropathy model. Taken together, these results offer preclinical proof of concept for TAT\u2010265WT as a tractable agent to protect against nitrosative stress\u2010induced endothelial dysfunction in renal microvessels.\u2014Deng, Y., Cai, Y., Liu, L., Lin, X., Lu, P., Guo, Y., Han, M., Xu, G. Blocking Tyr265 nitration of protein phosphatase 2A attenuates nitrosative stress\u2013induced endothelial dysfunction in renal microvessels. FASEB J. 33, 3718\u20133730 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201800885rr","type":"journal-article","created":{"date-parts":[[2018,12,6]],"date-time":"2018-12-06T13:11:17Z","timestamp":1544101877000},"page":"3718-3730","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Blocking Tyr265 nitration of protein phosphatase 2A attenuates nitrosative stress\u2013induced endothelial dysfunction in renal microvessels"],"prefix":"10.1096","volume":"33","author":[{"given":"Yuanjun","family":"Deng","sequence":"first","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Cai","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lele","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueping","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Pingfan","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiyan","family":"Guo","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Han","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Xu","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00042.2012"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvq115"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707462104"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/9152732"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.3.C1064"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307446101"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1480"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112400200"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn246"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom5031284"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00225.2006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00526.2011"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.05.002"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2013.01.015"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"4030","DOI":"10.1021\/es500001q","article-title":"Arsenite oxidation initiated by the UV photolysis of nitrite and nitrate","volume":"48","author":"Kim D. 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