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Galectin\u20101, encoded by lectin, galactoside\u2010binding, soluble (\n                    <jats:italic>LGALS<\/jats:italic>\n                    )\n                    <jats:italic>1<\/jats:italic>\n                    , has attracted growing attention for its facilitatory role in angiogenesis and fibrosis through its modification of VEGFA and TGF\u2010\u03b2 receptor signaling pathways. We reveal galectin\u20101 involvement in the mouse model of laser\u2010induced choroidal neovascularization (CNV) and subretinal fibrosis, both of which represent the pathogenesis of age\u2010related macular degeneration (AMD). Neither deletion nor overexpression of\n                    <jats:italic>Lgals1<\/jats:italic>\n                    affected physiologic retinal development or visual function. Galectin\u20101\/\n                    <jats:italic>Lgals1<\/jats:italic>\n                    was upregulated by CNV induction, whereas deletion of\n                    <jats:italic>Lgals1<\/jats:italic>\n                    suppressed CNV together with downstream molecules of VEGF receptor (VEGFR)2. Loss of\n                    <jats:italic>Lgals1<\/jats:italic>\n                    also attenuated subretinal fibrosis, expression of epithelial\u2010mesenchymal transition (EMT) markers including\n                    <jats:italic>Snai1<\/jats:italic>\n                    , and phosphorylation of SMAD family member 2. Supporting these\n                    <jats:italic>in vivo<\/jats:italic>\n                    findings, silencing of\n                    <jats:italic>LGALS1<\/jats:italic>\n                    in human retinal pigment epithelial (RPE) cells inhibited TGF\u2010\u03b21\u2010induced EMT\u2010related molecules and cell motilities. Conversely, overexpression of\n                    <jats:italic>Lgals1<\/jats:italic>\n                    enhanced CNV and subretinal fibrosis. Specimens from patients with AMD demonstrated colocalization of galectin\u20101 with VEGFR2 in neovascular endothelial cells and with phosphorylated SMAD2 in RPE cells. These results suggested a biologic significance of galectin\u20101 as a key promotor for both angiogenesis and fibrosis in eyes with AMD.\u2014Wu, D., Kanda, A., Liu, Y., Kase, S., Noda, K., Ishida, S. Galectin\u20101 promotes choroidal neovascularization and subretinal fibrosis mediated\n                    <jats:italic>via<\/jats:italic>\n                    epithelialmesenchymal transition. FASEB J. 33, 2498\u20132513 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201801227r","type":"journal-article","created":{"date-parts":[[2018,10,2]],"date-time":"2018-10-02T15:49:40Z","timestamp":1538495380000},"page":"2498-2513","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Galectin\u20101 promotes choroidal neovascularization and subretinal fibrosis mediated\n                    <i>via<\/i>\n                    epithelialmesenchymal transition"],"prefix":"10.1096","volume":"33","author":[{"given":"Di","family":"Wu","sequence":"first","affiliation":[{"name":"Laboratory of Ocular Cell Biology and Visual Science Department of Ophthalmology Faculty of Medicine Graduate School of Medicine Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuhiro","family":"Kanda","sequence":"additional","affiliation":[{"name":"Laboratory of Ocular Cell Biology and Visual Science Department of Ophthalmology Faculty of Medicine Graduate School of Medicine Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Ocular Cell Biology and Visual Science Department of Ophthalmology Faculty of Medicine Graduate School of Medicine Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Kase","sequence":"additional","affiliation":[{"name":"Laboratory of Ocular Cell Biology and Visual Science Department of Ophthalmology Faculty of Medicine Graduate School of Medicine Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kousuke","family":"Noda","sequence":"additional","affiliation":[{"name":"Laboratory of Ocular Cell Biology and Visual Science Department of Ophthalmology Faculty of Medicine Graduate School of Medicine Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Ishida","sequence":"additional","affiliation":[{"name":"Laboratory of Ocular Cell Biology and Visual Science Department of Ophthalmology Faculty of Medicine Graduate School of Medicine Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(13)70145-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.9.081307.164350"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2015.05.042"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31030"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2012.07.038"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2013.10.019"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2013.02.012"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39104"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3758"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1835"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/2001-1326-3-23"},{"key":"e_1_2_8_13_1","first-page":"1222","article-title":"Transcriptional factors associated with epithelial-mesenchymal transition in choroidal neovascularization","volume":"17","author":"Hirasawa M.","year":"2011","journal-title":"Mol. 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