{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T22:38:46Z","timestamp":1779143926279,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,8,31]],"date-time":"2018-08-31T00:00:00Z","timestamp":1535673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Br1744\/11-2"],"award-info":[{"award-number":["Br1744\/11-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010127","name":"J\u00fcrgen Manchot Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010127","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recently,\n                    <jats:italic>Entamoeba histolytica<\/jats:italic>\n                    clones derived from isolate HM\u20101:IMSS that differ in their pathogenicity were identified. Whereas some clones induce amoebic liver abscesses (ALAs) in animal models of amoebiasis, others provoke only minimal liver lesions. Based on transcriptome studies of pathogenic and nonpathogenic clones, differentially expressed genes associated with reduced or increased liver pathology can be identified. Here, to analyze the influence of these genes on ALA formation in more detail, an RNA interference\u2010trigger mediated silencing approach was used. Using newly identified trigger sequences, the expression of 15 genes was silenced. The respective transfectants were analyzed for their ability to induce liver destruction in the murine model for the disease. Silencing of\n                    <jats:italic>EHI_180390<\/jats:italic>\n                    (encoding an AIG1 protein) increased liver pathology induced by a nonpathogenic parent clone, whereas silencing of\n                    <jats:italic>EHI_127670<\/jats:italic>\n                    (encoding a hypothetical protein) decreased the pathogenicity of an initially pathogenic parent clone. Additional phenotypical\n                    <jats:italic>in vitro<\/jats:italic>\n                    analyses of\n                    <jats:italic>EHI_127670<\/jats:italic>\n                    silencing as well as overexpression transfectants indicated that this molecule has an influence on size, growth, and cysteine peptidase activity of\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    . This work describes an example of how the sole operational method for effective gene silencing in\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    can be used for comprehensive analyses of putative pathogenicity factors.\u2014Matthiesen, J., Lender, C., Haferkorn, A., Fehling, H., Meyer, M., Matthies, T., Tannich, E., Roeder, T., Lotter, H., Bruchhaus, I. Trigger\u2010induced RNAi gene silencing to identify pathogenicity factors of\n                    <jats:italic>Entamoeba histolytica<\/jats:italic>\n                    . FASEB J. 33, 1658\u20131668 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201801313r","type":"journal-article","created":{"date-parts":[[2018,8,31]],"date-time":"2018-08-31T16:02:54Z","timestamp":1535731374000},"page":"1658-1668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Trigger\u2010induced RNAi gene silencing to identify pathogenicity factors of\n                    <i>Entamoeba histolytica<\/i>"],"prefix":"10.1096","volume":"33","author":[{"given":"Jenny","family":"Matthiesen","sequence":"first","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinna","family":"Lender","sequence":"additional","affiliation":[{"name":"Bernhard Nocht 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