{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T04:17:58Z","timestamp":1788841078087,"version":"build-2803163510"},"reference-count":70,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T00:00:00Z","timestamp":1558396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/2.0\/"}],"funder":[{"DOI":"10.13039\/501100004953","name":"Atomic Energy of Canada Limited","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004953","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mitochondria are highly dynamic organelles that respond rapidly to a number of stressors to regulate energy transduction, cell death signaling, and reactive oxygen species generation. We hypothesized that mitochondrial remodeling, comprising both structural and functional alterations, following ionizing radiation (IR) may underlie some of the tenets of radiobiology. Mesenchymal stem cells (MSCs) are precursors of bone marrow stroma and are altered in acute myeloid leukemia and by radiation and chemotherapy. Here, we report on changes in mitochondrial remodeling in human MSCs following X\u2010ray IR. Mitochondrial function was significantly increased in MSCs 4 h after IR as measured by mitochondrial oxygen consumption. Consistent with this elevated functional effect, electron transport chain supercomplexes were also increased in irradiated samples. In addition, mitochondria were significantly, albeit modestly, elongated, as measured by high\u2010throughput automated confocal imaging coupled with automated mitochondrial morphometric analyses. We also demonstrate in fibroblasts that mitochondrial remodeling is required for the adaptation of cells to IR. To determine novel mechanisms involved in mitochondrial remodeling, we performed quantitative proteomics on isolated mitochondria from cells following IR. Label\u2010free quantitative mitochondrial proteomics revealed notable changes in proteins in irradiated samples and identified prosaposin, and potentially its daughter protein saposin\u2010B, as a potential candidate for regulating mitochondrial function following IR. Whereas research into the biologic effects of cellular irradiation has long focused on nuclear DNA effects, our experimental work, along with that of others, is finding that mitochondrial effects may have broader implications in the field of stress adaptation and cell death in cancer (including leukemia) and other disease states.\u2014Patten, D. A., Ouellet, M., Allan, D. S., Germain, M., Baird, S. D., Harper, M.\u2010E., Richardson, R. B. Mitochondrial adaptation in human mesenchymal stem cells following ionizing radiation. FASEB J. 33, 9263\u20139278 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201801483rr","type":"journal-article","created":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T15:35:50Z","timestamp":1558452950000},"page":"9263-9278","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Mitochondrial adaptation in human mesenchymal stem cells following ionizing radiation"],"prefix":"10.1096","volume":"33","author":[{"given":"David A.","family":"Patten","sequence":"first","affiliation":[{"name":"Radiobiology and Health Branch Chalk River Laboratories Canadian Nuclear Laboratories (CNL)  Chalk River Ontario Canada"},{"name":"Faculty of Medicine Department of Biochemistry Microbiology, and Immunology University of Ottawa  Ottawa Ontario Canada"},{"name":"Ottawa Institute of Systems Biology  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Ouellet","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie M\u00e9dicale Universit\u00e9 du Qu\u00e9bec \u00e0 Trois\u2010Rivi\u00e8res  Trois\u2010Rivi\u00e8res Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Allan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Biochemistry Microbiology, and Immunology University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Germain","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie M\u00e9dicale Universit\u00e9 du Qu\u00e9bec \u00e0 Trois\u2010Rivi\u00e8res  Trois\u2010Rivi\u00e8res Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen D.","family":"Baird","sequence":"additional","affiliation":[{"name":"Children's Hospital of Eastern Ontario Research Institute  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary\u2010Ellen","family":"Harper","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Biochemistry Microbiology, and Immunology University of Ottawa  Ottawa Ontario Canada"},{"name":"Ottawa Institute of Systems Biology  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard B.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Radiobiology and Health Branch Chalk River Laboratories Canadian Nuclear Laboratories (CNL)  Chalk River Ontario Canada"},{"name":"McGill Medical Physics Unit Glen Site Cedars Cancer Centre  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,21]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"An Introduction to Radiobiology","author":"Mas A. 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