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The current study was conducted with the aim of investigating the role of exosomes from bone marrow\u2010derived mesenchymal stem cells (BM\u2010MSCs) carrying microRNA\u2010224\u20103p (miR\u2010224\u20103p) in traumatic ONFH. Initially, a microarray analysis was performed to screen the differentially expressed genes and miRs associated with traumatic ONFH. Patients with traumatic and nontraumatic ONFH were enrolled, and HUVECs were obtained. The BM\u2010MSCs\u2010derived exosomes were purified and characterized, after which HUVECs were cocultured with exosomes. The functional role of miR\u2010224\u20103p in traumatic ONFH was determined using ectopic expression, depletion, and reporter assay experiments. Endothelial cell proliferation, migration, invasion abilities, and angiogenesis were evaluated. Based on microarray analysis, miR\u2010224\u20103p was found to be down\u2010regulated, whereas focal adhesion kinase family interacting protein of 200 kDa (FIP200) was up\u2010regulated in ONFH. Traumatic ONFH exosomes resulted in the up\u2010regulation of FIP200 and down\u2010regulation of miR\u2010224\u20103p. FIP200 was confirmed to be a target gene of miR\u2010224\u20103p. Exosomes were internalized by vascular endothelial cells. The down\u2010regulation of exosomal miR\u2010224\u20103p was observed to promote endothelial cell proliferation, migration, invasion abilities, angiogenesis, and FIP200 expression. In addition, FIP200 overexpression promoted angiogenesis. In summary, the results highly indicated that lower miR\u2010224\u20103p levels in exosomes derived from BM\u2010MSCs promote angiogenesis of traumatic ONFH by up\u2010regulating FIP200. The present study provides a potential strategy for the treatment of traumatic ONFH.\u2014Xu, H.\u2010J., Liao, W., Liu, X.\u2010Z., Hu, J., Zou, W.\u2010Z., Ning, Y., Yang, Y., Li, Z.\u2010H. Down\u2010regulation of exosomal microRNA\u2010224\u20103p derived from bone marrow\u2010derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head. FASEB J. 33, 8055\u20138068 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201801618rrr","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T14:21:17Z","timestamp":1554819677000},"page":"8055-8068","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Down\u2010regulation of exosomal microRNA\u2010224\u20103p derived from bone marrow\u2013derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head"],"prefix":"10.1096","volume":"33","author":[{"given":"Hai-Jia","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Wuhan Third Hospital Tongren Hospital Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Wuhan Third Hospital Tongren Hospital Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Zhong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Wuhan Third Hospital Tongren Hospital Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Hu","sequence":"additional","affiliation":[{"name":"Wuhan Sports University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Zhong","family":"Zou","sequence":"additional","affiliation":[{"name":"Wuhan Sports University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Ning","sequence":"additional","affiliation":[{"name":"Hubei University of Chinese Medicine  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Wuhan Sports University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhang-Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Wuhan Third Hospital Tongren Hospital Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000489684"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25812"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.F.00964"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.appet.2016.05.008"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000480181"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000329502"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.5435\/JAAOS-22-07-455"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-010-1009-5"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2016.02.061"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2011.10.002"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2005231"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013247"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000447878"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.7808"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000329978"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20097"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22079"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33229"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5871"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg405"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","unstructured":"Smyth G. 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