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The dichotomous effect of TGF\u2010\u03b2 on epithelial\u2010mesenchymal transition (EMT) between trophoblast invasion and cancer progression remains unknown and a critical concern. We attenuated the expression of TGF\u2010\u03b2 type 1 receptor (coding by\n                    <jats:italic>TGFBR1<\/jats:italic>\n                    ) with RNA interference in trophoblastic cells and significantly enhanced the trophoblastic invasion. Analysis of micro\u2010RNA profiles in trophoblasts indicated microRNA\u20107 as a key molecule linking TGF\u2010\u03b2 with the negative regulation of trophoblast invasion. We then attenuated\n                    <jats:italic>TGFBR1<\/jats:italic>\n                    and\n                    <jats:italic>miR\u20107<\/jats:italic>\n                    transcription by transducing either short hairpin RNA targeting\n                    <jats:italic>TGFBR1<\/jats:italic>\n                    or anti\u2013\n                    <jats:italic>miR<\/jats:italic>\n                    \u2010\n                    <jats:italic>7<\/jats:italic>\n                    \u2010locked nucleonic acid, and we observed an up\u2010regulation of EMT\u2010related transcription factors (TFs) and their downstream effectors, causing a mesenchymal transition of trophoblasts. Conversely, overexpression of\n                    <jats:italic>TGFBR1<\/jats:italic>\n                    or\n                    <jats:italic>miR\u20107<\/jats:italic>\n                    led to the epithelial transition of trophoblasts. Our results showed that TGF\u2010\u03b2\u2013induced\n                    <jats:italic>miR\u20107<\/jats:italic>\n                    expression negatively modulated the TGF\u2010\u03b2\u2013SMAD family member 2\u2010mediated EMT pathway\n                    <jats:italic>via<\/jats:italic>\n                    targeting EMT\u2010related TFs and down\u2010regulating their mesenchymal markers. These findings possibly explain, at least in part, why TGF\u2010\u03b2 exerts an opposite effect on EMT during trophoblast invasion and cancer progression.\u2014Shih, J.\u2010C., Lin, H.\u2010H., Hsiao, A.\u2010C., Su, Y.\u2010T., Tsai, S., Chien, C.\u2010L., Kung, H.\u2010N. Unveiling the role of microRNA\u20107 in linking TGF\u2010\u03b2\u2013Smad\u2010mediated epithelial\u2010mesenchymal transition with negative regulation of trophoblast invasion. FASEB J. 33, 6281\u20136295 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201801898rr","type":"journal-article","created":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T13:29:19Z","timestamp":1550755759000},"page":"6281-6295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Unveiling the role of microRNA\u20107 in linking TGF\u2010\u03b2\u2010Smad\u2010mediated epithelial\u2010mesenchymal transition with negative regulation of trophoblast invasion"],"prefix":"10.1096","volume":"33","author":[{"given":"Jin\u2010Chung","family":"Shih","sequence":"first","affiliation":[{"name":"Graduate Institute of Anatomy and Cell Biology College of Medicine National Taiwan University  Taipei Taiwan"},{"name":"Graduate Institute of Medical Genomics and Proteomics 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