{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T22:08:11Z","timestamp":1761948491572,"version":"build-2065373602"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T00:00:00Z","timestamp":1551312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R01DE016652"],"award-info":[{"award-number":["1R01DE016652"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["1R15DE024313"],"award-info":[{"award-number":["1R15DE024313"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Salivary glands are a major component of the mucosal immune system that confer adaptive immunity to mucosal pathogens. As previously demonstrated, immunization of the submandibular gland with tissue culture\u2013derived murine cytomegalovirus (tcMCMV) or replication\u2010deficient adenoviruses expressing individual murine cytomegalovirus (MCMV) genes protected mice against a lethal MCMV challenge. Here, we report that salivary gland inoculation of BALB\/cByJ mice with tcMCMV or recombinant adenoviruses differentially activates T helper (T\n                    <jats:sub>h<\/jats:sub>\n                    )1, \u20102, and \u201017 cells in the salivary glands\n                    <jats:italic>vs<\/jats:italic>\n                    . the associated lymph nodes. After inoculation with tcMCMV, lymphocytes from the submandibular gland preferentially express the transcription factor T\u2010cell\u2013specific T\u2010box transcription factor (T\u2010bet), which controls the expression of the hallmark T\n                    <jats:sub>h<\/jats:sub>\n                    1 cytokine, IFN\u2010\u03b3. Lymphocytes from the periglandular lymph nodes (PGLNs) express both T\u2010bet and GATA\u2010binding protein 3 (GATA3), which promotes the secretion of IL\u20104, \u20105, and \u201010 from T\n                    <jats:sub>h<\/jats:sub>\n                    2 cells. In contrast, after inoculation with replication\u2010deficient adenoviruses, lymphocytes from the submandibular gland express T\u2010bet, GAT A3, and RAR\u2010related orphan receptor \u03b3, thymus\u2010specific isoform (ROR\/\u03b3t) (required for differentiation of T\n                    <jats:sub>h<\/jats:sub>\n                    17 cells) and forkhead box P3 (Foxp3) (required for the differentiation of regulatory T cells). Lymphocytes from the PGLNs were not activated. The differential induction of T\n                    <jats:sub>h<\/jats:sub>\n                    responses in the salivary gland\n                    <jats:italic>vs<\/jats:italic>\n                    . the PGLNs after inoculation with attenuated virus\n                    <jats:italic>vs<\/jats:italic>\n                    . a nominal protein antigen supports the use of the salivary as an alternative mucosal route for administering vaccines.\u2014Liu, G., Zhang, F., Wang, R., London, S. D., London, L. Salivary gland immunization\n                    <jats:italic>via<\/jats:italic>\n                    Wharton's duct activates differential T\u2010cell responses within the salivary gland immune system. FASEB J. 33, 6011\u20136022 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201801993r","type":"journal-article","created":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T15:51:08Z","timestamp":1551369068000},"page":"6011-6022","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Salivary gland immunization\n                    <i>via<\/i>\n                    Wharton's duct activates differential T\u2010cell responses within the salivary gland immune system"],"prefix":"10.1096","volume":"33","author":[{"given":"Guangliang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Oral Biology and Pathology School of Dental Medicine Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangfang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Pathology School of Dental Medicine Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruixue","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Pathology School of Dental Medicine Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven D.","family":"London","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Pathology School of Dental Medicine Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucille","family":"London","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Pathology School of Dental Medicine Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2009.04.002"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.12.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1777"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.12.034"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-174656"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2006.12.010"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-244178"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.05.010"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.50"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2741\/e777"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6548-9_2"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101212"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2017.02.003"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2016.06.010"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-16-0193"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2016.07.003"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2017.0027"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2017.08.001"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40794-017-0047-z"},{"key":"e_1_2_8_21_1","unstructured":"Brune W. 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