{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:14:49Z","timestamp":1761707689759,"version":"build-2065373602"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T00:00:00Z","timestamp":1586390400000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T00:00:00Z","timestamp":1554768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB1140033"],"award-info":[{"award-number":["MCB1140033"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM112793","R01GM130858"],"award-info":[{"award-number":["R01GM112793","R01GM130858"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cellular checkpoints controlling entry into mitosis monitor the integrity of the DNA and delay mitosis onset until the alteration is fully repaired. However, this canonical response can weaken, leading to a spontaneous bypass of the checkpoint, a process referred to as checkpoint adaptation. Here, we have investigated the contribution of microcephalin 1 (MCPH1), mutated in primary microcephaly, to the decatenation checkpoint, a less\u2010understood G\n                    <jats:sub>2<\/jats:sub>\n                    pathway that delays entry into mitosis until chromosomes are properly disentangled. Our results demonstrate that, although MCPH1 function is dispensable for activation and maintenance of the decatenation checkpoint, it is required for the adaptive response that bypasses the topoisomerase II inhibition\u2013mediated G\n                    <jats:sub>2<\/jats:sub>\n                    arrest. MCPH1, however, does not confer adaptation to the G\n                    <jats:sub>2<\/jats:sub>\n                    arrest triggered by the ataxia telangiectasia mutated\u2014and ataxia telangiectasia and rad3 related\u2010based DNA damage checkpoint. In addition to revealing a new role for MCPH1 in cell cycle control, our study provides new insights into the genetic requirements that allow cellular adaptation to G\n                    <jats:sub>2<\/jats:sub>\n                    checkpoints, a process that remains poorly understood.\u2014Arroyo, M., Kuriyama, R., Guerrero, I., Keifenheim, D., Ca\u00f1uelo, A., Calahorra, J., S\u00e1nchez, A., Clarke, D. J., Marchai, J. A. MCPH1 is essential for cellular adaptation to the G\n                    <jats:sub>2<\/jats:sub>\n                    \u2010phase decatenation checkpoint. FASEB J. 33,8363\u20138374 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802009rr","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T14:54:02Z","timestamp":1554821642000},"page":"8363-8374","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["MCPH1 is essential for cellular adaptation to the G\n                    <sub>2<\/sub>\n                    \u2010phase decatenation checkpoint"],"prefix":"10.1096","volume":"33","author":[{"given":"Mar\u00eda","family":"Arroyo","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental Universidad de Ja\u00e9n  Ja\u00e9n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoko","family":"Kuriyama","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cell Biology, and Development University of Minnesota\u2013Minneapolis  Minneapolis Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Israel","family":"Guerrero","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n y Formaci\u00f3n Agraria y Pesquera (IFAPA Centro El Toru\u00f1o)  El Puerto de Santa Mar\u00eda Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Keifenheim","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cell Biology, and Development University of Minnesota\u2013Minneapolis  Minneapolis Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Carmelo","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental Universidad de Ja\u00e9n  Ja\u00e9n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Calahorra","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental Universidad de Ja\u00e9n  Ja\u00e9n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental Universidad de Ja\u00e9n  Ja\u00e9n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duncan J.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cell Biology, and Development University of Minnesota\u2013Minneapolis  Minneapolis Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Alberto","family":"Marchai","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental Universidad de Ja\u00e9n  Ja\u00e9n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.07.015"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81870-X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2016.1237769"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/339518"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/422855"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2342"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-12793-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1431"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200810159"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.22.3471"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.9.24.14157"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.302"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2013.04.017"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C400139200"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.07.002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1116815"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705245200"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210595"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040387"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.4.7.10032"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.367"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603537"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/372467a0"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221430898"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1828"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00412-015-0512-2"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.9.8.11470"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004120000065"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206109200"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00678-3"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200405167"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200401139"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e10-02-0125"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.068254"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.17.3200"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.232"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210402"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11693-014-9151-9"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.286351.116"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.3.2412"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6685"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201802009RR","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201802009RR","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201802009RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:20:07Z","timestamp":1761690007000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201802009RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,9]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1096\/fj.201802009RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.201802009rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"type":"print","value":"0892-6638"},{"type":"electronic","value":"1530-6860"}],"subject":[],"published":{"date-parts":[[2019,4,9]]},"assertion":[{"value":"2018-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}