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Tranylcypromine (TCP), an irreversible inhibitor of monoamine oxidase (MAO), has been used as an antidepressant and anxiolytic agent in the clinical treatment of mood and anxiety disorders. TCP has been discovered to exert anabolic effect on osteoblasts, and MAO\u2010A has also been verified as an important mediator in prostate cancer cells to accelerate osteoclastogenesis. In current study, we were focused on TCP and MAO\u2010A effects on osteoclastogenesis. As illustrated by tartrate\u2010resistant acid phosphatase staining, TCP was capable of inhibiting osteoclastogenesis induced by receptor activators of the NF\u2010\u03baB ligand (RANKL) in bone marrow\u2010derived macrophage cells without any cytotoxicity. It was also shown to effectively suppress bone resorption of OCs. The subsequent study revealed that TCP inhibited osteoclastogenesis\u2010related genes in a time\u2010dependent manner through protein kinase B (AKT)\u2013mediated mechanism followed by the nuclear factor of activated T cells, cytoplasmic 1 (NFATc1)\u2010c\u2010fos pathway. And TCP could overcome the osteoclastogenic effects of AKT activator SC79. In addition, our results indicated that the expression and catalytic activity of MAO\u2010A were up\u2010regulated by RANKL stimulation and down\u2010regulated by TCP\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Furthermore, the effects of MAO\u2010A knockdown on OC differentiation indicated that MAO\u2010A played an important role in osteoclastogenesis\n                    <jats:italic>in vitro<\/jats:italic>\n                    and might contribute to the inhibitory effects of TCP. And AKT, NFATc1, and c\u2010fos were involved in the MAO\u2010A pathway. Notably, our in vivo study reflected that TCPs were capable of restoring the bone loss in LPS\u2010induced calvaria osteolysis and estrogen deficiency\u2013induced osteoporosis models. Thus, our current work provided a potential option for the treatment of bone loss diseases and highlighted the important role of MAO\u2010A in osteoclastogenesis as well.\u2014Liu, Z., Yang, K., Yan, X., Wang, T., Jiang, T., Zhou, Q., Qi, J., Qian, N., Zhou, H., Chen, B., Huang, P., Guo, L., Zhang, X., Xu, X., Jiang, M., Deng, L. The effects of tranylcypromine on osteoclastogenesis\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . FASEB J. 33, 9828\u20139841 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802242rr","type":"journal-article","created":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T18:46:43Z","timestamp":1562784403000},"page":"9828-9841","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The effects of tranylcypromine on osteoclastogenesis\n                    <i>in vitro<\/i>\n                    and\n                    <i>in vivo<\/i>"],"prefix":"10.1096","volume":"33","author":[{"given":"Zhuochao","family":"Liu","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory for Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Shanghai Jiaotong University School of Medicine  Shanghai China"},{"name":"Department of Orthopedics 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