{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T20:37:38Z","timestamp":1775680658050,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T00:00:00Z","timestamp":1555372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R00-AR-064837"],"award-info":[{"award-number":["R00-AR-064837"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Periostin (\n                    <jats:italic>POSTN<\/jats:italic>\n                    ), a secretory matricellular matrix protein, plays a multitude of biologic functions. Various splice variants of\n                    <jats:italic>POSTN<\/jats:italic>\n                    have been described; however, their expression pattern and functional implications are not completely understood. This study was undertaken to decipher the differential expression pattern of\n                    <jats:italic>POSTN<\/jats:italic>\n                    and its splice variants in various tissues and cell types. We show that\n                    <jats:italic>POSTN<\/jats:italic>\n                    was more highly expressed in anterior cruciate ligament (ACL) remnants compared with articular cartilage at the cellular and tissue level. Isoforms 1 and 8 were highly expressed only in articular chondrocytes, suggesting their splice\u2010specific regulation in chondrocytes. To discern the role of total\n                    <jats:italic>POSTN<\/jats:italic>\n                    and full\u2010length human POSTN isoform 1 (\n                    <jats:italic>hPOSTN\u2010001<\/jats:italic>\n                    ), we stably transfected human chondrosarcoma 1 (hCh\u20101) cell line with\n                    <jats:italic>hPOSTN\u2010001<\/jats:italic>\n                    using a pcDNA3.1\u2013hPOSTN\u2010001 construct. RNA\u2010sequencing analysis of hCh\u20101 cells identified differentially expressed genes with a known role in chondrocyte function and osteoarthritis. Similar expression of a subset of candidate genes was revealed in ACL progenitor cells and chondrocytes as well as in ACL progenitor cells in which\n                    <jats:italic>POSTN<\/jats:italic>\n                    activity was altered by overexpression and by small interfering RNA gene knockdown. Cells expressing total\n                    <jats:italic>POSTN<\/jats:italic>\n                    , not isoform 1, exhibited increased cell adhesion potential. These findings suggest an important role for\n                    <jats:italic>POSTN in<\/jats:italic>\n                    the knee.\u2014Cai, L., Brophy, R. H., Tycksen, E. D., Duan, X., Nunley, R. M., Rai, M. F. Distinct expression pattern of periostin splice variants in chondrocytes and ligament progenitor cells. FASEB J. 33, 8386\u20138405 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802281r","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T20:31:18Z","timestamp":1555446678000},"page":"8386-8405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Distinct expression pattern of periostin splice variants in chondrocytes and ligament progenitor cells"],"prefix":"10.1096","volume":"33","author":[{"given":"Lei","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Musculoskeletal Research Center Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert H.","family":"Brophy","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Musculoskeletal Research Center Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric D.","family":"Tycksen","sequence":"additional","affiliation":[{"name":"Genome Technology Access Center Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Musculoskeletal Research Center Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan M.","family":"Nunley","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Musculoskeletal Research Center Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Farooq","family":"Rai","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Musculoskeletal Research Center Washington University School of Medicine  St. Louis Missouri USA"},{"name":"Department of Cell Biology and Physiology Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M708029200"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2940271"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20115"},{"key":"e_1_2_8_5_1","first-page":"161","article-title":"Role of alternative splicing of periostin in human bladder carcinogenesis","volume":"32","author":"Kim C. J.","year":"2008","journal-title":"Int. J. Oncol."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.7.1239"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-10-30"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-6-80"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071297"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21633"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.05.035"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4137\/JCM.S5899"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1515\/bmc-2016-0004"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8701386"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21224"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.7A7321.2007"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2018.01.017"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-015-0682-3"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23661"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546516643810"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2017.12.004"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2014.10.014"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070624"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100160502"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.K.00919"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts356"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks042"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv412"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-161"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/986048"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt285"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.15-272427"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-012-0689-y"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096526"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11552-009-9164-0"},{"key":"e_1_2_8_40_1","first-page":"6744","article-title":"Differential gene expression between normal and tumor-derived ovarian epithelial cells","volume":"60","author":"Ismail R. S.","year":"2000","journal-title":"Cancer Res."},{"key":"e_1_2_8_41_1","first-page":"593","article-title":"Overexpression of periostin is significantly correlated to the tumor angiogenesis and poor prognosis in patients with esophageal squamous cell carcinoma","volume":"7","author":"Wang W.","year":"2014","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(04)00081-9"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7819-12-287"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147837"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.088690"},{"key":"e_1_2_8_46_1","first-page":"1759","article-title":"Opposite regulation of epithelial-to-mesenchymal transition and cell invasiveness by periostin between prostate and bladder cancer cells","volume":"38","author":"Kim C. J.","year":"2011","journal-title":"Int. J. Oncol."},{"key":"e_1_2_8_47_1","doi-asserted-by":"crossref","first-page":"3205","DOI":"10.2147\/OTT.S163626","article-title":"Periostin overexpression is associated with worse prognosis in nasopharyngeal carcinoma from endemic area: a cohort study","volume":"11","author":"Wei Y. C.","year":"2018","journal-title":"OncoTargets Ther."},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-017-3134-7"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-497"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102727200"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2015.00147"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.120121"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm7120535"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-015-1255-5"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.10.5547"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2006.04.008"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1494-y"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12120"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2007.10.001"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201802281R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201802281R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201802281R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:20:24Z","timestamp":1761690024000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201802281R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,16]]},"references-count":58,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1096\/fj.201802281R"],"URL":"https:\/\/doi.org\/10.1096\/fj.201802281r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,16]]},"assertion":[{"value":"2018-10-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}