{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,8]],"date-time":"2026-11-08T03:39:10Z","timestamp":1794109150549,"version":"4.8.0"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T00:00:00Z","timestamp":1559692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100014718","name":"Innovative Research Group Project of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31801172"],"award-info":[{"award-number":["31801172"]}],"id":[{"id":"10.13039\/100014718","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The leucine\u2010rich repeat kinase 2 (LRRK2) is expressed in various immune cells and involved in regulating inflammatory processes. LRRK2 facilitates calcium extrusion exchanger and sodium\u2010calcium exchanger activity and hence influences intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration in dendritic cells (DCs). DC maturation and migration are governed by the intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration, but the related mechanisms whereby LRRK2 regulates DC function and involved Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels are still under investigation. In the previous study, we found that LRRK2\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    DCs exhibited higher store\u2010operated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    entry (SOCE) activity than LRRK2\n                    <jats:sup>+\/+<\/jats:sup>\n                    DCs. Herein, we ascertained the exact SOCE components by using genetic, pharmacological, and fluorescent approaches. Ca\n                    <jats:sup>2+<\/jats:sup>\n                    imaging showed that LRRK2 kinase activity negatively modulated SOCE activity. Moreover, LRRK2 deficiency resulted in an enhanced migration capacity of DCs but had little effect on the maturation process. SOCE is widely known to regulate DC functions; we wanted to dissect the reason why LRRK2 specifically influenced DC migration and therefore silenced ORAI1, ORAI2, and ORAI3, respectively. Transwell assays showed that both ORAI1 and ORAI2 silencing markedly decreased the migration of DCs, but only ORAI1 deficiency influenced the expression of maturation markers CD11c, CD86, and major histocompatibility complex class II. Of note, LRRK2 deficiency increased ORAI2 expression but not that of ORAI1 and ORAI3. Thus, we suggest that LRRK2 modulates DC migration by interfering with ORAI2.\u2014Yan, J., Zhao, W., Gao, C., Liu, X., Zhao, X., Wei, T., Gao, Z. Leucine\u2010rich repeat kinase 2 regulates mouse dendritic cell migration by ORAI2. FASEB J. 33, 9775\u20139784 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802550r","type":"journal-article","created":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T16:29:31Z","timestamp":1559752171000},"page":"9775-9784","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Leucine\u2010rich repeat kinase 2 regulates mouse dendritic cell migration by ORAI2"],"prefix":"10.1096","volume":"33","author":[{"given":"Jing","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Physiology Jining Medical University  Jining China"},{"name":"Department of Physiology Xinxiang Medical University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology Huaian Nursing College  Huaian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Physiology Jining Medical University  Jining China"},{"name":"Department of Physiology Xinxiang Medical University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology Jining Medical University  Jining China"},{"name":"Department of Physiology Xinxiang Medical University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuliang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Physiology Jining Medical University  Jining China"},{"name":"Department of Physiology Xinxiang Medical University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Physiology Jining Medical University  Jining China"},{"name":"Department of Physiology Xinxiang Medical University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"And Zhaodi","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Physiology Jining Medical University  Jining China"},{"name":"Department of Physiology Xinxiang Medical University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-264028"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1073858418756309"},{"key":"e_1_2_8_4_1","first-page":"5577","article-title":"LRRK2 is involved in the IFN\u2010gamma response and host response to pathogens. 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