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It has been suggested that mutations in the\n                    <jats:italic>DNM2<\/jats:italic>\n                    gene, associated with 3 inherited diseases, disrupt endocytosis. However, how exactly mutations affect the nanoscale morphology of endocytic machinery has never been studied. In this paper, we used live correlative scanning ion conductance microscopy (S1CM) and fluorescence confocal microscopy (FCM) to study how disease\u2010associated mutations affect the morphology and kinetics of clathrin\u2010coated pits (CCPs) by directly following their dynamics of formation, maturation, and internalization in skin fibroblasts from patients with centronuclear myopathy (CNM) and in Cos\u20107 ce1ls expressing corresponding dynamin mutants. Using S1CM\u2010FCM, which we have deve1oped, we show how p.R465W mutation disrupts pit structure, preventing its maturation and internalization, and significantly increases the lifetime of CCPs. Differently, p.R522H slows down the formation of CCPs without affecting their internalization. We also found that CNM mutations in\n                    <jats:italic>DNM2<\/jats:italic>\n                    affect the distribution of caveoli and reduce dorsal ruffling in human skin fibroblasts. Collective1y, our S1CM\u2010FCM findings at single CCP leve1, backed up by electron microscopy data, argue for the impairment of several forms of endocytosis in\n                    <jats:italic>DNM2<\/jats:italic>\n                    \u2010linked CNM.\u2014Ali, T., Bednarska, J., Vassilopoulos, S., Tran, M., Diakonov, I. A., Ziyadeh\u2010Isleem, A., Guicheney, P., Gorelik, J., Korchev, Y. E., Reilly, M. M., Bitoun, M., Shevchuk, A. Correlative SICM\u2010FCM reveals changes in morphology and kinetics of endocytic pits induced by disease\u2010associated mutations in dynamin. FASEB J. 33, 8504\u20138518 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802635r","type":"journal-article","created":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T15:01:19Z","timestamp":1556118079000},"page":"8504-8518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Correlative SICM\u2010FCM reveals changes in morphology and kinetics of endocytic pits induced by disease\u2010associated mutations in dynamin"],"prefix":"10.1096","volume":"33","author":[{"given":"Tayyibah","family":"Ali","sequence":"first","affiliation":[{"name":"Division of Experimental Medicine Department of Medicine Imperial College London  London United 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