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HIF\u20101 and HIF\u20102 regulate endothelial cell (EC) adaptation by activating genesignaling cascades that promote endothelial migration, growth, and differentiation. An HIF\u20101 to HIF\u20102 transition or switch governs this process from acute to prolonged hypoxia. In the present study, we evaluated the mechanisms governing the HIF switch in 10 different primary human ECs from different vascular beds during the early stages of hypoxia. The studies demonstrate that the switch from HIF\u20101 to HIF\u20102 constitutes a universal mechanism of cellular adaptation to hypoxic stress and that\n                    <jats:italic>HIF1A<\/jats:italic>\n                    and\n                    <jats:italic>HIF2A<\/jats:italic>\n                    mRNA stability differences contribute to HIF switch. Furthermore, using 4 genome\u2010wide mRNA expression arrays of HUVECs during normoxia and after 2, 8, and 16 h of hypoxia, we show using bioinformatics analyses that, although a number of genes appeared to be regulated exclusively by HIF\u20101 or HIF\u20102, the largest number of genes appeared to be regulated by both.\u2014Bartoszewski, R., Moszynska, A., Serocki, M., Cabaj, A., Polten, A., Ochocka, R., Dell'Italia, L., Bartoszewska, S., Kr\u00f3liczewski, J., Dabrowski, M., Collawn, J. F. Primary endothelial cell\u2013specific regulation of hypoxia\u2010inducible factor (HIF)\u20101 and HIF\u20102 and their target gene expression profiles during hypoxia. FASEB J. 33, 7929\u20137941 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802650rr","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T15:26:32Z","timestamp":1553700392000},"page":"7929-7941","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":197,"title":["Primary endothelial cell\u2013specific regulation of hypoxia\u2010inducible factor (HIF)\u20101 and HIF\u20102 and their target gene expression profiles during hypoxia"],"prefix":"10.1096","volume":"33","author":[{"given":"Rafal","family":"Bartoszewski","sequence":"first","affiliation":[{"name":"Department of Biology and Pharmaceutical Botany Medical University of Gdansk  Gdansk 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