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The transcription factor scleraxis marks vertebrate tendons from early specification.\n                    <jats:italic>Scleraxis<\/jats:italic>\n                    \u2010null mice are viable and have a range of tendon and bone defects in the trunk and limbs but no described cranial phenotype. We report the expression of zebrafish scleraxis orthologs: scleraxis homolog (\n                    <jats:italic>scx<\/jats:italic>\n                    )\n                    <jats:italic>\u2010a<\/jats:italic>\n                    and\n                    <jats:italic>scxb<\/jats:italic>\n                    in cranial and intramuscular tendons and in other skeletal elements. Single mutants for either\n                    <jats:italic>scxa<\/jats:italic>\n                    or\n                    <jats:italic>scxb<\/jats:italic>\n                    , generated by clustered regularly interspaced short palindromic repeats (CRISPR)\/CRISPR\u2010associated protein 9 (Cas9), are viable and fertile as adult fish. Although\n                    <jats:italic>scxb<\/jats:italic>\n                    mutants show no obvious phenotype,\n                    <jats:italic>scxa<\/jats:italic>\n                    mutant embryos have defects in cranial tendon maturation and muscle misalignment. Mutation of both scleraxis genes results in more severe defects in cranial tendon differentiation, muscle and cartilage dysmorphogenesis and paralysis, and lethality by 2\u20135 wk, which indicates an essential function of scleraxis for craniofacial development. At juvenile and adult stages, ribs in\n                    <jats:italic>scxa<\/jats:italic>\n                    mutants fail to mineralize and\/or are small and heavily fractured.\n                    <jats:italic>Scxa<\/jats:italic>\n                    mutants also have smaller muscle volume, abnormal swim movement, and defects in bone growth and composition. Scleraxis function is therefore essential for normal craniofacial form and function and vital for fish development.\u2014Kague, E., Hughes, S. M., Lawrence, E. A., Cross, S., Martin\u2010Silverstone, E., Hammond, C. L., Hinits, Y. Scleraxis genes are required for normal musculoskeletal development and for rib growth and mineralization in zebrafish. FASEB J. 33, 9116\u20139130 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802654rr","type":"journal-article","created":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T13:20:17Z","timestamp":1558099217000},"page":"9116-9130","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Scleraxis genes are required for normal musculoskeletal development and for rib growth and mineralization in zebrafish"],"prefix":"10.1096","volume":"33","author":[{"given":"Erika","family":"Kague","sequence":"first","affiliation":[{"name":"Department of Physiology Pharmacology, and Neuroscience, Medical Sciences University of Bristol  Bristol United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon M.","family":"Hughes","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics School of Basic and Medical Biosciences Faculty of Life Sciences and Medicine King's College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Lawrence","sequence":"additional","affiliation":[{"name":"Department of Physiology Pharmacology, and Neuroscience, Medical Sciences University of Bristol  Bristol United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Cross","sequence":"additional","affiliation":[{"name":"Department of Physiology Pharmacology, and Neuroscience, Medical Sciences University of Bristol  Bristol United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Martin\u2010Silverstone","sequence":"additional","affiliation":[{"name":"Department of Physiology Pharmacology, and Neuroscience, Medical Sciences University of Bristol  Bristol United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chrissy L.","family":"Hammond","sequence":"additional","affiliation":[{"name":"Department of Physiology Pharmacology, and Neuroscience, Medical Sciences University of Bristol  Bristol United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaniv","family":"Hinits","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics School of Basic and Medical Biosciences Faculty of Life Sciences and Medicine King's College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.047498"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.114777"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.151266"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.19.3855"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.20049"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-004-0694-9"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00268-X"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.4.1099"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21179"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1002\/dvdy.21718","article-title":"Properties of branchiomeric and somite\u2010derived muscle development in Tbx1 mutant embryos","volume":"237","author":"Grif one R.","year":"2008","journal-title":"Dev. 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