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Here, we report the efficient conversion of C\u2010to\u2010T or G\u2010to\u2010A in\n                    <jats:italic>X. tropicalis<\/jats:italic>\n                    using the rat apolipoprotein B mRNA editing enzyme catalytic subunit 1\u2013XTEN\u2010clustered regularly interspaced short palindromic repeat\u2010associated protein 9 (Cas9) nickase\u2010uracil DNA glycosylase inhibitor\u2010nuclear localization sequence base editor [base editor 3 (BE3)]. Coinjection of guide RNA and the Cas9 mutant complex mRNA into 1\u2010cell stage\n                    <jats:italic>X. tropicalis<\/jats:italic>\n                    embryos caused precise C\u2010to\u2010T or G\u2010to\u2010A substitution in 14 out of 19 tested sites with efficiencies of 5\u201375%, which allowed for easy establishment of stable lines. Targeting the conserved T\u2010box 5 R237 and Tyr C28 residues in\n                    <jats:italic>X. tropicalis<\/jats:italic>\n                    with the BE3 system mimicked human Holt\u2010Oram syndrome and oculocutaneous albinism type 1A, respectively. Our data indicate that BE3 is an easy and efficient tool for precise base editing in\n                    <jats:italic>X. tropicalis<\/jats:italic>\n                    .\u2014Shi, Z., Xin, H., Tian, D., Lian, J., Wang, J., Liu, G., Ran, R., Shi, S., Zhang, Z., Shi, Y., Deng, Y., Hou, C., Chen, Y. Modeling human point mutation diseases in\n                    <jats:italic>Xenopus tropicalis<\/jats:italic>\n                    with a modified CRISPR\/Cas9 system. FASEB J. 33,6962\u20136968 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802661r","type":"journal-article","created":{"date-parts":[[2019,3,7]],"date-time":"2019-03-07T15:27:23Z","timestamp":1551972443000},"page":"6962-6968","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Modeling human point mutation diseases in\n                    <i>Xenopus tropicalis<\/i>\n                    with a modified CRISPR\/Cas9 system"],"prefix":"10.1096","volume":"33","author":[{"given":"Zhaoying","family":"Shi","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Southern University of Science and Technology Shenzhen China"},{"name":"Shenzhen Key Laboratory of Cell Microenvironment Department 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingru","family":"Lian","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Southern University of Science and Technology Shenzhen China"},{"name":"Shenzhen Key Laboratory of Cell Microenvironment Department of Biology Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhui","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Southern University of Science and Technology Shenzhen China"},{"name":"Shenzhen Key Laboratory of Cell Microenvironment Department of Biology Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Southern University of Science and Technology Shenzhen China"},{"name":"Shenzhen Key Laboratory of Cell Microenvironment Department of Biology Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rensen","family":"Ran","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Southern University of Science and Technology Shenzhen China"},{"name":"Shenzhen Key Laboratory of Cell Microenvironment Department of Biology Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songyuan","family":"Shi","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Southern University of Science and Technology Shenzhen China"},{"name":"Shenzhen Key Laboratory of Cell Microenvironment Department of Biology Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Southern University of Science and Technology Shenzhen China"},{"name":"Shenzhen Key Laboratory of Cell Microenvironment Department of Biology Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Children's Hospital of Chongqing Medical University Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Children's Hospital of Chongqing Medical University Chongqing China"},{"name":"Chongqing Key Laboratory of Pediatrics Children's 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