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Previous works showed that mechanical loading stimulated bone formation and accelerated fracture healing. Focusing on the role of Wnt3a (wingless\/integrated 3a), this study was aimed to assess effects of mechanical loading to the spine, using ovariectomized (OVX) mice as a model of osteoporosis. Two\u2010week daily application of this novel loading (4N, 10Hz, 5 min\/d) altered bone remodeling with an increase in Wnt3a. Spinal loading promoted osteoblast differentiation, endothelial progenitor cell migration, and tube formation and inhibited osteoclast formation, migration, and adhesion. A transient silencing of Wnt3a altered the observed loading effects. Spinal loading significantly increased bone mineral density, bone mineral content, and bone area per tissue area. The loaded OVX group showed a significant increase in the number of osteoblasts and reduction in osteoclast surface\/bone surface. Though expression of osteoblastic genes was increased, the levels of osteoclastic genes were decreased by loading. Spinal loading elevated a microvascular volume as well as VEGF expression. Collectively, this study supports the notion that Wnt3a\u2010mediated signaling involves in the effect of spinal loading on stimulating bone formation, inhibiting bone resorption, and promoting angiogenesis in OVX mice. It also suggests that Wnt3a might be a potential therapeutic target for osteoporosis treatment. \u2014Li, X., Liu, D., Li, J., Yang, S., Xu, J., Yokota, H., Zhang, P. Wnt3a involved in the mechanical loading on improvement of bone remodeling and angiogenesis in a postmenopausal osteoporosis mouse model. FASEB J. 33, 8913\u20138924 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802711r","type":"journal-article","created":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T15:49:04Z","timestamp":1556120944000},"page":"8913-8924","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Wnt3a involved in the mechanical loading on improvement of bone remodeling and angiogenesis in a postmenopausal osteoporosis mouse model"],"prefix":"10.1096","volume":"33","author":[{"given":"Xinle","family":"Li","sequence":"first","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University  Tianjin China"},{"name":"Key Laboratory of Hormones and Development (Ministry of Health) Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University  Tianjin China"},{"name":"Key Laboratory of Hormones and Development (Ministry of Health) Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinfeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Yokota","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Indiana University Purdue University  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University  Tianjin China"},{"name":"Key Laboratory of Hormones and Development (Ministry of Health) Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University  Tianjin China"},{"name":"Department of Biomedical Engineering Indiana University Purdue University  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,24]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"5139","article-title":"Nanotechnology for treating osteoporotic vertebral fractures","volume":"10","author":"Gao C.","year":"2015","journal-title":"Int. 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