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Several studies imply that the loop region of SNAP25 plays important roles in SNARE\u2010complex assembly. However, why and how the flexible loop facilitates the complex assembly remains poorly understood because it is purposely deleted in almost all structural studies. By using NMR spectroscopy and circular dichroism spectropolarimetry, we characterized SNAP25 structure and interactions with other SNAREs in aqueous buffer and in the membrane. We found that the N\u2010terminal of the SNAP25 loop region binds with membrane, and this interaction induced a disorder\u2010to\u2010order conformational change of the loop, resulting in enhanced interaction between the C\u2010terminal of the SNAP25 loop and syx\u20101. We further proved that SNARE\u2010complex assembly efficiency decreased when we disrupted the electrostatic interaction between C\u2010terminal of the SNAP25 loop and syx\u20101, suggesting that the SNAP25 loop region facilitates SNARE\u2010complex assembly through promoting prefusion SNARE binary complex formation. Our work elucidates the role of the flexible loop and the membrane environment in SNARE\u2010complex assembly at the residue level, which helps to understand membrane fusion, a fundamental transport and communication process in cells.\u2014Jiang, X., Zhang, Z., Cheng, K., Wu, Q., Jiang, L., Pielak, G. J., Liu, M., Li, C. Membrane\u2010mediated disorder\u2010to\u2010order transition of SNAP25 flexible linker facilitates its interaction with syntaxin\u20101 and SNARE\u2010complex assembly. FASEB J. 33, 7985\u20137994 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201802796r","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T15:59:02Z","timestamp":1553702342000},"page":"7985-7994","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Membrane\u2010mediated disorder\u2010to\u2010order transition of SNAP25 flexible linker facilitates its interaction with syntaxin\u20101 and SNARE\u2010complex assembly"],"prefix":"10.1096","volume":"33","author":[{"given":"Xin","family":"Jiang","sequence":"first","affiliation":[{"name":"Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in Wuhan Wuhan National Laboratory for Optoelectronics Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences  Wuhan China"},{"name":"Graduate University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in Wuhan Wuhan National Laboratory for Optoelectronics Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Cheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in Wuhan Wuhan National Laboratory for Optoelectronics Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in Wuhan Wuhan National Laboratory for Optoelectronics Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in Wuhan Wuhan National Laboratory for Optoelectronics Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences  Wuhan China"},{"name":"Graduate University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary J.","family":"Pielak","sequence":"additional","affiliation":[{"name":"Department of Chemistry Department of Biochemistry and Biophysics University of North Carolina\u2013Chapel Hill  Chapel Hill North Carolina USA"},{"name":"Lineberger Comprehensive Cancer Center University of North Carolina\u2013Chapel Hill  Chapel Hill North Carolina USA"},{"name":"Integrative Program for Biological and Genome Sciences University of North Carolina\u2013Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maili","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in Wuhan Wuhan National Laboratory for 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