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For instance, the mechanistic target of rapamycin (mTOR) is reduced during protein malnutrition and is known to be modulated by concentrations of several amino acids when in a multiprotein signaling complex that contains regulatory\u2010associated protein of mTOR. We hypothesized that a partial decrease in mTOR complex 1 (mTORC1) activity intrinsic to B\u2010lineage cells would perturb lymphocyte development or function, or both. We show that a cell\u2010intrinsic decrease in mTORC1 activity impacted developmental progression, antigen receptor repertoire, and function along the B lineage. Thus, preimmune repertoires of B\u2010lineage cells were altered in the marrow and periphery in a genetic model of regulatory\u2010associated protein of mTOR haploinsufficiency. An additional role for mTORC1 was revealed when a B\u2010cell antigen receptor transgene was found to circumvent the abnormal B\u2010cell development: haploinsufficient B cells were profoundly impaired in responses to antigen\n                    <jats:italic>in vivo.<\/jats:italic>\n                    Collectively, our findings indicate that mTORC1 serves as a rheostat that shapes differentiation along the B lineage, the preimmune repertoire, and antigen\u2010driven selection of mature B cells. The findings also reveal a range in the impact of this nutrient sensor on activity\u2010response relationships for distinct endpoints.\u2014Raybuck, A. L., Lee, K., Cho, S. H., Li, J., Thomas, J. W., Boothby, M. R. mTORC1 as a cell\u2010intrinsic rheostat that shapes development, preimmune repertoire, and function of B lymphocytes. FASEB J. 33, 13202\u201313215 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900069r","type":"journal-article","created":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T18:07:07Z","timestamp":1568830027000},"page":"13202-13215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["mTORC1 as a cell\u2010intrinsic rheostat that shapes development, preimmune repertoire, and function of B lymphocytes"],"prefix":"10.1096","volume":"33","author":[{"given":"Ariel L.","family":"Raybuck","sequence":"first","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology Vanderbilt University Medical Center Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keunwook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Hallym University Chuncheon Gangwon\u2010do South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Hoon","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology Vanderbilt University Medical Center Nashville Tennessee USA"},{"name":"Vanderbilt Institute for Infection, Immunology, and Inflammation (VI4) Vanderbilt University Medical Center Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology Vanderbilt University Medical Center Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Medicine Vanderbilt University Medical Center Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark R.","family":"Boothby","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology Vanderbilt University Medical Center Nashville Tennessee USA"},{"name":"Vanderbilt Institute for Infection, Immunology, and Inflammation (VI4) Vanderbilt University Medical Center Nashville Tennessee USA"},{"name":"Department of Medicine Vanderbilt University Medical Center Nashville Tennessee USA"},{"name":"Department of Cancer Biology Vanderbilt University Medical Center Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/34.10.2117"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4804(86)90211-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2009.08.026"},{"key":"e_1_2_8_5_1","first-page":"220","article-title":"Nutrition and the immune response \u2013 a review","volume":"49","author":"Dreizen S.","year":"1979","journal-title":"Int. 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